动物营养学报2025，Vol.37Issue(2)：1221-1233,13.DOI:10.12418/CJAN2025.106
基于子宫黏膜转录组研究调控蛋鸡产蛋后期蛋壳质量相关的基因和信号通路
Study of Key Genes and Signaling Pathways Regulating Laying Hens'Eggshell Quality in Late Laying Period Based on Transcriptome Sequencing of Uterine
摘要
Abstract
This study aimed to identify important candidate genes and signaling pathways associated with egg-shell quality by comparing the mechanical property,ultrastructure of higher and lower quality eggshells as well as the uterine morphology and gene expression of laying hens.Twelve Hy-Line brown laying hens aged 80-weeks were selected in the experiment,and 6 hens with higher eggshell quality[with(3.54±0.11)kg/cm2 eggshell strength and(385.06±12.46)mm eggshell thickness]were selected as the high quality group(HQE group),and 6 hens with lower eggshell quality[with(2.54±0.07)kg/cm2 eggshell strength and(256.17±18.36)mm eggshell thickness)]were selected as the low quality group(LQE group).Each group collected 6 eggs a day for 5 days.After weighing of the egg,the eggshell strength was measured with an eggshell strength meter,the eggshell thickness was determined by a spiral micrometer,and then eggshell ratio was calculated;the ultrastructure of eggshell such as the effective layer thickness,mammillary layer thickness and mammillary width were observed with a scanning electron microscope,and the mammillary thickness layer ratio was calcu-lated;histological section technique was used to compare the uterine morphological differences between two groups;RNA-seq technology was employed to compare the differentially expressed genes(DEGs)of uterine samples between the two groups,and these DEGs were analyzed for GO and KEGG enrichment.The results showed that,in comparison with the LQE group,the eggshell strength,eggshell thickness,eggshell weight and eggshell ratio in HQE group were significantly increased(P＜0.05),as well as the eggshell effective layer thickness,mammillary layer thickness and mammillary layer thickness ratio(P＜0.05).In respect to uterine morphology,the mucosal tubular gland cells of uterine in HQE group were more tightly arranged,and the height of the villi of the uterine mucosa was significantly higher than in LQE group(P＜0.05).The LQE group was selected as the control group,and 303 DEGs were identified,including 103 up-regulated DEGs and 200 down-regulated DEGs.GO analysis suggested the DEGs were mainly enriched in DNA replication and immune response and other related biological processes.KEGG analysis indicated enriched pathways primarily associat-ed with immune and inflammatory response,including cytokine-cytokine receptor interaction,Th17 cell differ-entiation,tumor necrosis factor(TNF)and Toll-like receptor signaling pathways,among which signal Trans-ducer and activator of transcription 1(STAT1),CC chemokine receptor 2(CCR2),CC chemokine ligand 4(CCL4),CC chemokine ligand 20(CCL20)and interleukin 18 receptor 1(IL18R1)were repeatedly en-riched in these pathways,and these genes were significantly down-regulated in the HQE group.From the above results,it can be concluded that the deterioration of mechanical properties and ultrastructure of eggshell in late laying period was accompanied by the deterioration of uterine tissue morphology.By functional analysis,signa-ling pathways such as cytokine-cytokine receptor interaction in uterine mucosa might play an important role in the process of eggshell mineralization by regulating the expression of genes related to uterine immune and in-flammatory responses.These results provided theoretical basis for improving eggshell quality in the late laying period of hens.[Chinese Journal of Animal Nutrition,2025,37(2):1221-1233]关键词
蛋鸡/产蛋后期/蛋壳/转录组测序/差异表达基因/信号通路Key words
laying hens/late laying period/eggshell/RNA-seq/differentially expressed gene/signaling pathway分类
农业科技引用本文复制引用
邵丹,瞿源,刘良基,童海兵..基于子宫黏膜转录组研究调控蛋鸡产蛋后期蛋壳质量相关的基因和信号通路[J].动物营养学报,2025,37(2):1221-1233,13.基金项目
现代农业产业技术体系专项资金项目(CARS-40-S23) （CARS-40-S23）
