动物营养学报2025，Vol.37Issue(2)：1234-1244,11.DOI:10.12418/CJAN2025.107
芜菁多糖对黄羽鹌鹑外周血淋巴细胞免疫和抗氧化功能的影响
Effects of Brassica rapa L.Polysaccharides on Immune and Antioxidant Functions of Peripheral Blood Lymphocytes in Yellow-Feathered Quails
摘要
Abstract
This study aimed to evaluate the effects of Brassica rapa L.polysaccharides(BRP)on the immune and antioxidant functions of peripheral blood lymphocytes(PBLs)in yellow-feathered quails.The Brassica ra-pa L.roots were crushed,and BRP was extracted using hot water extraction,macroporous resin adsorption,and DEAE-52 cellulose column purification,resulting in a purity of 99.85％and a molecular weight of 118 ku.Blood samples were taken from the veins of yellow-feathered quails,and lymphocytes were isolated with a PBLs extraction kit.Lymphocytes were suspended in RPMI-1640 medium(with 10％fetal bovine serum and 1％double antibiotics),and the cell concentration was adjusted to 1×107 cells/mL using Trypan blue staining and counting for PBLs culture in vitro test.The PBLs culture in vitro test involved five concentrations(0,25,50,100 and 200 μg/mL)of BRP,with six replicates for each concentration.After 24 hours of PBLs culture,and the supernatant was collected to measure immune indexes[tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-8(IL-8),interleukin-10(IL-10),immunoglobulin A(IgA),immuno-globulin G(IgG)and immunoglobulin M(IgM)contents]and antioxidant indexes[glutathione peroxidase(GSH-Px),superoxide dismutase(SOD),catalase(CAT)activities and malondialdehyde(MDA)con-tent].Real-time quantitative PCR(RT-qPCR)method was also performed to assess the mRNA relative ex-pression levels of immune-related genes,including IL-1β,IL-8,IL-10,TNF-α,Toll-like receptor 4(TLR4),nuclear factor-κB(NF-κB),myeloid differentiation factor 88(MyD88)and antioxidant-related genes,inclu-ding GSH-Px,CAT,SOD.The results showed as follows:1)in terms of immune function,compared with the blank control group(0 μg/mL BRP),BRP at the concentrations of 50,100 and 200 μg/mL extremely signif-icantly increased IgA and IgM contents in the supernatant(P＜0.01),while extremely significantly reduced IL-1β,IL-8 and TNF-α contents in the supernatant(P＜0.01);moreover,BRP at the concentrations of 50 and 100 μg/mL also significantly increased IgG content in the supernatant(P＜0.05).2)In terms of antioxi-dant function,compared with the blank control group,BRP extremely significantly increased the supernatant CAT activity at the concentrations of 50,100 and 200 μg/mL(P＜0.01);furthermore,the mRNA relative expression levels of TLR4,NF-κB,IL-8 and TNF-α in the supernatant were extremely significantly reduced by BRP at the concentrations of 50,100 and 200 μg/mL(P＜0.01),whereas the mRNA relative expression lev-els of SOD,GSH-Px and CAT were significantly or extremely significantly elevated by BRP at the concentra-tions of 100 and 200 μg/mL(P＜0.05 or P＜0.01),and the mRNA relative expression level of MyD88 was extremely significantly elevated by BRP at the concentrations of 25 and 50 μg/mL(P＜0.0l).In conclusion,BRP enhances the immune and antioxidant functions of PBLs in yellow-feathered quails by modulating lympho-cyte responses,with 100 μg/mL being the optimal concentration under this experimental condition.[Chinese Journal of Animal Nutrition,2025,37(2):1234-1244]关键词
芜菁多糖/黄羽鹌鹑/外周血淋巴细胞/免疫/抗氧化/NF-κB信号通路Key words
Brassica rapa L.polysaccharides/yellow-feathered quails/peripheral blood lymphocytes/im-mune/antioxidant/NF-κB signal pathway分类
农业科技引用本文复制引用
朱建军,柳婷婷,王政力,马艳,申红,王俊刚..芜菁多糖对黄羽鹌鹑外周血淋巴细胞免疫和抗氧化功能的影响[J].动物营养学报,2025,37(2):1234-1244,11.基金项目
国家自然科学基金项目(31660703) （31660703）
