动物营养学报2025，Vol.37Issue(2)：1245-1259,15.DOI:10.12418/CJAN2025.108
植物多酚与硝基化合物协同降低体外瘤胃甲烷排放的作用研究
Research on Synergistic Reduction of Methane Emissions from Ruminants by Plant Polyphenols and Nitro-Compounds
摘要
Abstract
This study aimed to investigate the mechanism by which plant polyphenols and nitro-compounds re-duce rumen methane(CH4)emissions using an in vitro approach.Through in vitro rumen simulation,the stud-y explores the effects of plant polyphenols in combination with nitro-compounds on gas production,fermenta-tion parameters,and microbial composition during in vitro fermentation,as well as the synergistic mechanisms between the two in terms of their effects on rumen fermentation and methane suppression.The experiment is di-vided into four groups:control group(CON group),supplemented with 50 mg/g of procyanidins(PC)(PC group),supplemented with 10 mmol/L of nitroethanol(NEOH)(NEOH group),and supplemented with 25 mg/g of PC+5 mmol/L of NEOH(MIX group),with 10 fermentation bottles in each group and a fer-mentation duration of 24 h.The results showed as follows:1)compared with CON group,the total gas pro-duction of MIX group was significantly decreased(P＜0.05),and the CH4 production of NEOH group and MIX group was significantly decreased(P＜0.05).2)Compared with CON group,the molar ratio of isobutyr-ic acid and isovaleric acid in rumen and the ratio of ethylene to propylene in PC group were significantly in-creased(P＜0.05),the molar ratio of propionic acid was significantly decreased(P＜0.05),the molar ratio of acetic acid and the ratio of ethylene to propylene in NEOH group and MIX group were significantly decreased(P＜0.05),and the molar ratio of propionic acid was significantly increased(P＜0.05).3)At the bacterial genus level,there were significant differences in 10 microorganisms among the groups.Specifically,compared to the CON group,the relative abundance of Prevotella significantly decreased in the PC and NEOH groups(P＜0.05)but did not change significantly in the MIX group(P＞0.05).The relative abundance of Succini-vibrionaceae_UCG-002 significantly decreased in the PC group(P＜0.05)but did not change significantly in the NEOH and MIX groups(P＞0.05).The relative abundance of Ruminobacter significantly increased in the NEOH and MIX groups(P＜0.05)but did not change significantly in the PC group(P＞0.05).At the archaeal genus level,there were significant differences in five microorganisms,including the relative abundance of Methanobrevibacter(P＜0.05),among the groups.Specifically,compared to the CON group,the relative a-bundance of Methanobrevibacter significantly increased in the NEOH,PC and MIX groups(P＜0.05).The relative abundance of Methanosphaera did not change significantly in the NEOH and PC groups(P＞0.05)but significantly decreased in the MIX group(P＜0.05).4)LEfSe analysis results showed that at the bacterial ge-nus level,three microorganisms such as Prevotella were significantly enriched in the CON group,while 13 mi-croorganisms such as Christensenellaceae_R-7_group,Lachnospiraceae_NK3A20_group,and Lachnospiraceae_NK4A214_group were significantly enriched in the PC group.Four microorganisms such as Succinivibrio and Selenomonas were significantly enriched in the NEOH group,and four microorganisms such as Succinivibrionaceae_UCG-002 and Ruminococcus were significantly enriched in the MIX group.At the ar-chaeal genus level,three microorganisms such as Methanosphaera were significantly enriched in the CON group,while one marker species was significantly enriched in the PC group.Methanobrevibacter was signifi-cantly enriched in the MIX group.The results indicate that the addition of PC and NEOH can affect the rumen flora structure.The NEOH and MIX alter the rumen fermentation pattern,shifting from acetate production to propionate production,thereby reducing CH4 production,and there is a synergistic effect between the two.[Chinese Journal of Animal Nutrition,2025,37(2):1245-1259]关键词
植物多酚/硝基化合物/瘤胃/甲烷/16S rRNAKey words
plant polyphenols/nitro-compounds/rumen/methane/16S rRNA分类
农业科技引用本文复制引用
谭健,张靖,杨东峰,韩赛波,余诗强,赵慧颖,蒋林树,赵玉超,郭刚..植物多酚与硝基化合物协同降低体外瘤胃甲烷排放的作用研究[J].动物营养学报,2025,37(2):1245-1259,15.基金项目
家畜产业技术体系北京市创新团队(BAIC05-2022) （BAIC05-2022）
北京首农食品集团有限公司"乳业全产业链"绿色数智"技术集成创新与产业化应用"(SNSPKJ-2022) （SNSPKJ-2022）
