动物营养学报2025，Vol.37Issue(2)：1260-1270,11.DOI:10.12418/CJAN2025.109
枯草芽孢杆菌对黄曲霉毒素B1引起羊人工瘤胃异常发酵的改善作用
Improvement Effects of Bacillus subtilis on Abnormal Fermentation in Artificial Rumen Induced by Aflatoxin B1 in Sheep
摘要
Abstract
This experiment was conducted to investigate the effects of different doses of Bacillus subtilis supple-mentation on abnormal fermentation in artificial rumen induced by aflatoxin B1(AFB,)in sheep.Using a single factor experimental design and total mixed ration(TMR)as fermentation substrate,the experiment was divided into 7 groups,including control group(NC group),AFB,group and 5 experimental groups.The NC group was supplemented with 1 mL methanol,the AFB1 group was supplemented with 1 mL 10 μg/mL AFB1,and the experimental groups were supplemented with 1 mL 10 μg/mL AFB,and Bacillus subtilis preparations of 0.01％,0.03％,0.06％,0.12％and 0.24％,respectively,with 3 replicates in each group.On the day of the experiment,the fresh rumen fluid from 3 slaughtered sheep was randomly collected and cultured at 39 ℃ for 4,8,12,24 and 48 h according to the experimental design.pH and gas production were recorded,the con-tents of ammonia nitrogen(NH3-N),microbial protein(MCP)and volatile fatty acid(VFA)were deter-mined,and the microbiota was detected by fluorescence quantitative PCR.The results showed as follows:1)At 24 h of fermentation,the gas production in AFB,group was significantly decreased compared with NC group(P＜0.05),and the gas production in 0.03％group was extremely significantly increased compared with AFB1 group(P＜0.01).2)At 24 h of fermentation,there was no significant difference in pH in fermentation broth among all groups(P＞0.05).The contents of NH3-N and MCP in AFB1 group were lower than those in NC group,but there was no significant difference(P＞0.05);the contents of NH3-N and MCP in 0.03％group were extremely significantly higher than those in AFB1 group(P＜0.01).3)At 24 h of fermentation,the each VFA content in fermentation broth in AFB1 group was numerically lower than that in NC group,and the isobutyrate content was significantly lower than that in NC group(P＜0.05);the contents of acetate,buty-rate and total volatile fatty acid(TVFA)in 0.03％group were extremely significantly increased compared with AFB1 group(P＜0.01),and the contents of propionate and isobutyrate were significantly increased compared with AFB1 group(P＜0.05).4)Compared with NC group,the relative abundances of Fibrobacter succino-genes,Ruminobacter amylophilus and protozoa in AFB1 group were extremely significantly decreased(P＜0.01),and the relative abundances of Butyrivibrio fibrisolvens and Prevotella ruminicola were significantly de-creased(P＜0.05).Compared with AFB1 group,the relative abundances of Ruminococcus albus,Ruminococ-cus flavefaciens,Prevotella ruminicola and methanogens in 0.03％group was significantly increased(P＜0.05),and the relative abundances of Butyrivibrio fibrisolvens,Ruminobacter amylophilus and protozoa were extremely significantly increased(P＜0.01).In conclusion,AFB1 can reduce the rumen gas production and contents of NH3-N,MCP and VFA in vitro fermentation,and inhibit rumen microbial activity;dietary supple-mentation of 0.03％Bacillus subtilis can effectively alleviate the inhibitory effects of artificial rumen fermenta-tion induced by AFB1 and promote rumen fermentation.[Chinese Journal of Animal Nutrition,2025,37(2):1260-1270]关键词
枯草芽孢杆菌/黄曲霉毒素B1/瘤胃/体外发酵/微生物区系Key words
Bacillus subtilis/aflatoxin B1/rumen/in vitro fermentation/microbiota分类
农业科技引用本文复制引用
张照杰,姬中豪,皇甫和平,王金明,韩一铭,高宇凤,任晓丽,宋超,李志娟,李洪政,石冬梅,贾雨婷..枯草芽孢杆菌对黄曲霉毒素B1引起羊人工瘤胃异常发酵的改善作用[J].动物营养学报,2025,37(2):1260-1270,11.基金项目
新一轮河南省重点学科(学科名称:兽医 （）
学科序号:312) （）
河南省科技攻关项目(232102111037) （232102111037）
