动物营养学报2025，Vol.37Issue(2)：1286-1297,12.DOI:10.12418/CJAN2025.111
槲皮素对高铜诱导的小鼠卵巢氧化损伤的缓解作用分析
Analysis of Alleviating Effect of Quercetin Against High Copper Induced Ovarian Oxidative Damage in Mice
摘要
Abstract
This experiment aimed to investigate the alleviating effect of quercetin on high copper-induced oxi-dative damage in mouse ovaries.Female ICR mice were used and randomly divided into six groups,and each group had seven mice.Mice in the control group were gavaged with 0.5％carboxymethylcellulose sodium(CMC-Na)+orally administered purified water every day,mice in the high copper group were orally adminis-tered 200 mg/kg copper sulfate pentahydrate(CuSO4·5H2O)every day,mice in the quercetin group were gavaged with 100 mg/kg quercetin+orally administered purified water every day,mice in the low concentration of quercetin protection group were gavaged with 25 mg/kg quercetin+orally administered 200 mg/kg CuSO4·5H2O every day,mice in the medium concentration of quercetin protection group were gavaged with 50 mg/kg quercetin+orally administered 200 mg/kg CuSO4·5H2O every day,and mice in the high concen-tration of quercetin protection group were gavaged with 100 mg/kg quercetin+orally administered 200 mg/kg CuSO4·5H2O every day.During the experiment period,all mice were fed a basal diet for 7 days in the pre-liminary experiment and 28 days in the formal experiment.After the experiment,the body weight of the mice was recorded,the ovaries were weighed to calculate the ovarian coefficient,blood samples were collected to measure serum antioxidant stress indicators,the expression of oxidative stress-related genes in ovarian tissue was detected by real-time fluorescence quantitative PCR,the histological changes of the ovaries were observed by hematoxylin and eosin(HE)staining,and the ovarian tissue were observed for apoptosis by Tunel staining.The results showed as follows:1)compared with the control group,the ovarian coefficient of the high copper group decreased significantly(P＜0.05);compared with the high copper group,the ovarian coefficient was significantly increased after supplementation with 50 and 100 mg/kg of quercetin(P＜0.05).2)Histopatho-logical changes in ovarian tissues showed that follicular atresia and apoptotic cell number were obviously in-creased,and the connection between granulosa cells and oocytes was looser in the high copper group,and that after quercetin supplementation,the number of apoptotic cells and atretic follicles were decreased,especially mice supplemented with 50 mg/kg quercetin,the connection between granulosa cells and oocytes became tigh-ter,and apoptosis cell number was obviously reduced.3)Compared with the control group,the serum malon-dialdehyde(MDA)content of mice in the high copper group was extremely significantly increased(P＜0.01),while the serum total superoxide dismutase(T-SOD)and catalase(CAT)activities were significantly or ex-tremely significantly decreased(P＜0.05 or P＜0.01);the supplementation of 50 mg/kg quercetin extremely significantly reduced the serum MDA content which was increased by high copper(P＜0.01),and significantly or extremely significantly increased the serum CAT and T-SOD activities(P＜0.05 or P＜0.01).4)Compared with the control group,the mRNA relative expression levels of CAT(P＞0.05),superoxide dismutase 1(SOD1)(P＞0.05)and superoxide dismutase 2(SOD2)(P＜0.05)in ovarian tissues of the high copper group were decreased,whereas the mRNA relative expression level of heme oxygenase-1(HO-1)was in-creased(P＞0.05);the mRNA relative expression levels of CAT,SOD1,SOD2 and HO-1 did not change sig-nificantly(P＞0.05),but returned to normal level after supplementation with 50 or 100 mg/kg quercetin.In conclusion,the supplementation of 50 or 100 mg/kg quercetin can alleviate the oxidative damage induced by high copper in the ovaries of mice.[Chinese Journal of Animal Nutrition,2025,37(2):1286-1297]关键词
槲皮素/高铜/卵巢/氧化应激Key words
quercetin/high copper/ovary/oxidative stress分类
农业科技引用本文复制引用
邢文文,齐南南,葛方才,王彬彬,李梦轩,刘吉英..槲皮素对高铜诱导的小鼠卵巢氧化损伤的缓解作用分析[J].动物营养学报,2025,37(2):1286-1297,12.基金项目
国家自然科学基金项目(32202674) （32202674）
江苏省自然科学基金项目(BK20200994) （BK20200994）
