儿科药学杂志2025，Vol.31Issue(3)：14-21,8.DOI:10.13407/j.cnki.jpp.1672-108X.2025.03.004
基于数据挖掘和网络药理学探讨中药治疗儿童感染性腹泻的用药规律及作用机制
Medication Rules and Mechanism of Traditional Chinese Medicine in the Treatment of Infectious Diarrhea in Children Based on Data Mining and Network Pharmacology
摘要
Abstract
Objective:To probe into the medication rules and mechanism of traditional Chinese medicine in the treatment of infectious diarrhea in children based on date mining and network pharmacology.Methods:Modern literature data from databases such as CNKI,Wanfang,VIP,CBM and PubMed,as well as the experiences and medical cases related to infectious diarrhea in children recorded in the works of modem and contemporary physicians,were collected and screened.Traditional Chinese Medicine Inheritance Support Platform(V3.0)was used to analyze the rules of prescription drugs such as frequency,syndromes,four Qi and five flavors,meridian tropism,association rules,and core combinations.Drug targets were obtained from the HIT 2.0 database.The obtained targets were calculated by using CytoScape 3.10.2 software and mapped and intersected with the targets of infectious diarrhea in children screened from human gene database(GeneCards),DisGeNET and online mendelian inheritance in man(OMIM).The STRING and Cytoscape 3.10.2 software were used to construct a visual protein-protein interaction(PPI)network model for the intersection targets.The gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)enrichment analysis were performed for key targets by using the Weisengxin platform.Results:A total of 323 traditional Chinese medicine prescriptions in the treatment of infectious diarrhea in children were included.The properties and flavors were mainly warm and sweet,mainly attributed to the spleen and stomach meridians.The drugs with the highest usage frequency were respectively Poria cocos,Atractylodes macrocephala koidz,licorice,Codonopsis radix,Citrus reticulata.The high-frequency drug pair was Poria cocos-Atractylodes macrocephala koidz.Three groups of core drug combination were obtained through methods such as association rules and K-means clustering.There were 26 key targets of high-frequency drugs in the treatment of infectious diarrhea in children and 161 KEGG pathways were obtained.The key targets were epidermal growth factor receptor(EGFR),interleukin 6(IL-6),signal transduction and transcriptional activator 3(STAT3),tyrosine kinase receptor 2(ERBB2),and interferon gamma(IFN-γ),IL-10,transforming growth factor B1(TGFB1),signal transduction and transcriptional activator 1(STAT1),JANUS kinase 3(JAK3),forkhead box protein P3(FOXP3),tumor necrosis factor-related activator protein(CD40LG).And the main pathways were inflammatory bowel disease,Th7 cell differentiation,malaria,and JAK-STAT signaling pathway.Conclusion:The treatment method with"multiple components,multiple targets and multiple pathways"is the advantage of traditional Chinese medicine in the treatment of infectious diarrhea in children.The treatment of infectious diarrhea in children with traditional Chinese medicine takes strengthening and activating the spleen and stomach as the general principle,and is combined with methods such as clearing heat,draining dampness,promoting digestion,and removing stagnation.The core medications are Poria cocos and Atractylodes macrocephala koidz.Strengthening and activating the spleen and stomach runs through both deficiency syndromes and excess syndromes.The three new prescriptions discussed in this study may be related to inflammatory response,immune regulatory response,cell growth and apoptosis.关键词
儿童感染性腹泻/中药/数据挖掘/网络药理学/多靶点Key words
infectious diarrhea in children/traditional Chinese medicine/data mining/network pharmacology/multiple targets分类
医药卫生引用本文复制引用
陈建芬,李丽娟,梁倩莹,莫小兰,张刚,阮舒琪,李培武..基于数据挖掘和网络药理学探讨中药治疗儿童感染性腹泻的用药规律及作用机制[J].儿科药学杂志,2025,31(3):14-21,8.基金项目
国家自然科学基金项目,编号82003829 （）
国家中医药传承创新中心科研专项,编号2022ZD01 （）
广东省中医药局科研项目,编号20222163. （）
