福建农业科技2025，Vol.56Issue(1)：31-37,7.DOI:10.13651/j.cnki.fjnykj.2025.01.005
牛樟芝磷酸葡萄糖异构酶(PGI)基因的克隆、表达及蛋白特性分析
Analysis on the Cloning,Expression and Protein Characteristics of PGI Gene from Antrodia cinnamomea
摘要
Abstract
Glucose phosphate isomerase(PGI)catalyzes the conversion of 6-phosphate-glucose to 6-phosphate-fructose,which is an important branch node in the process of polysaccharide synthesis.By using PGI protein of Gano-derma lucidum as a probe,based on the genome database of Antrodia cinnamomea,the pgi gene of Antrodia cinnamo-mea was cloned by using the electronic cloning technology,and the characteristics of the protein encoded by the gene were analyzed.The transcript expression level of the gene during the fermentation process was detected by RT-PCR.The results showed that the cDNA length of pgi gene was 1 659 bp,encoding 552 amino acids,and the encoded PGI protein was hydrophilic and had no transmembrane and signal peptide structure.The secondary structure was domina-ted by 47.83%α-helix.The protein sequence had a close genetic relationship with Sparassis crispa and Amylocystis lapponica.In the process of solid culture and liquid culture of Antrodia cinnamomea,the level of pgi gene transcrip-tion expression increased with time,which was inversely proportional to the synthesis of polysaccharides.The results of this study could lay a foundation for the metabolic regulation of polysaccharide synthesis in Antrodia cinnamomea.关键词
牛樟芝/磷酸葡萄糖异构酶/多糖/蛋白特性Key words
Antrodia cinnamomea/Glucose phosphate isomerase/Polysaccharide/Protein characteristics分类
农业科学引用本文复制引用
刘宇煌,陆鑫,林娟娟,钟小丽,颜巧玲,蒋咏梅,章文贤..牛樟芝磷酸葡萄糖异构酶(PGI)基因的克隆、表达及蛋白特性分析[J].福建农业科技,2025,56(1):31-37,7.基金项目
福建省高校产学研联合创新项目(2022N5004). （2022N5004）
