福建农业科技2025，Vol.56Issue(1)：38-43,6.DOI:10.13651/j.cnki.fjnykj.2025.01.006
3种紫淮山茎尖脱毒组培快繁技术研究
Study on Stem Tip Detoxification and Tissue-cultured Rapid Propagation Technology of Three Kinds of Purple Yam
摘要
Abstract
The medium suitable for the stem tip induction,virus-free seedling proliferation and rooting of purple yam and the matrix for the transplantation and domestication of in vitro regenerated seedlings were screened,in order to provide healthy seedlings for the production.Three kinds of purple yam were used as the test materials,and different concentrations of 6-BA,NAA,KT and GA3 were added to MS medium,thus to study the effects of different concentration ratios of plant-growth regulator on the stem tip induction,subculture proliferation and rooting.Then,the in vitro regenerated seedlings were transplanted into the seedbeds of imported peat soil,perlite and coconut bran with different ratios,and the effects of different matrix ratios on the survival rate of transplanting were observed.The results showed that the shoot tip seedling rates of Wuyishan purple yam,Hutoushan purple yam and Zhenghe sweet potato were the highest in MS medium supplemented with 1.0 mg·L-1 6-BA+0.1 mg·L-1 NAA,which were 77.78%,77.78%and 71.11%,respectively.The proliferation coefficient of Wuyishan purple yam was the highest in MS medium supplemented with 1.0 mg·L-1 6-BA+0.5 mg·L-1 KT+0.3 mg·L-1 NAA+0.1 mg·L-1 GA3+0.2 g·L-1 AC,which was 4.6.The proliferation coefficients of Hutoushan purple yam and Zhenghe sweet potato were the highest in MS medium supplemented with 0.5 mg·L-1 6-BA+0.2 mg·L-1 KT+0.1 mg·L-1 NAA+0.1 mg·L-1 GA3+0.2 g·L-1 AC,which were 4.83 and 4.82,respectively.The root growth of Wuyishan purple yam,Hutoushan purple yam and Zhenghe sweet potato was the best in MS medium supplemented with 0.5 mg·L-1 IBA+0.5 mg·L-1 NAA+0.5 mg·L-1 CCC+0.5 g·L-1 AC.The transplanting survival rate of three kinds of purple yam was the highest when transplanted in 100%matrix of imported peat soil,which was 82.7%,80.0%and 82.0%,respectively.关键词
不同品种/紫淮山/茎尖脱毒/组培快繁Key words
Different varieties/Purple yam/Stem tip detoxification/Tissue culture and rapid propagation分类
园艺学与植物营养学引用本文复制引用
陈丹心..3种紫淮山茎尖脱毒组培快繁技术研究[J].福建农业科技,2025,56(1):38-43,6.基金项目
泉州市科技计划项目(2022N029). （2022N029）
