康复学报2025，Vol.35Issue(1)：44-50,7.DOI:10.3724/SP.J.1329.2025.01006
基于NRG1/ErbB4信号通路探讨针刺对孤独症谱系障碍行为学的影响
Exploring the Effects of Acupuncture on Behaviors of Autism Spectrum Disorder Based on the NRG1/ErbB4 Signaling Pathway
摘要
Abstract
Objective To explore the effect of acupuncture on the behavioral activities of autism spectrum disorder(ASD)model rats based on the recombinant neuregulin 1/receptor protein-tyrosine kinase erbB-4 signaling pathway and to investigate the possible mechanism by which acupuncture improves the behavioral activities of ASD.Methods Adult SPF-grade Wistar rats were used.The model was established by intraperitoneal injection of sodium valproate in pregnant female rats at 12.5 days of gestation.Twenty-four ASD model rats were randomly divided into model group,acupuncture group,and NRG1 group,with 8 rats in each group.The NRG1 group was obtained by lateral ventricle NRG1 lentivirus infection on the basis of the ASD model.The acupunc-ture group was treated at the"Zhi San Zhen"acupoints,with a depth of approximately 1 mm,and the needles were retained for 40 minutes and manipulated once every 10 minutes at each acupoint.The model group and the NRG1 group only simulated the grasp-ing action.The three groups were intervened at the same time every day,once a day,for 14 consecutive days.The behavioral activi-ties of the rats were observed by the three-chamber social test,the open field test,and the Morris water maze.The relative mRNA and protein expression levels of NRG1 and ErbB4 in the frontal lobe of the brain were detected by qPCR and Western blot methods,respectively.Results In the first stage,compared with the model group,the NRG1 group spent more time in contact with the unfa-miliar rat 1(P<0.05);compared with the model group,the acupuncture group and the NRG1 group spent less time in contact with the object(P<0.05).In the second stage,compared with the model group,the acupuncture group and the NRG1 group spent less time in contact with the unfamiliar rat 1(P<0.05);compared with the model group,the acupuncture group and the NRG1 group spent more time in contact with the unfamiliar rat 2(P<0.05).Compared with the model group,the exploration behavior,that is,the frequency of exploration in the middle area,increased in the acupuncture group and the NRG1 group(P<0.05);compared with the model group,the time spent in the middle area increased in the acupuncture group and the NRG1 group(P<0.05).In the spatial ex-ploration stage,compared with the model group,the exploration time in the target quadrant and the frequency of crossing the target platform in the NRG1 group increased significantly(P<0.05);compared with the model group,the total swimming distance in the acupuncture group and the NRG1 group decreased significantly(P<0.05).Compared with the model group,the relative expression levels of NRG1 and ErbB4 mRNA in the NRG1 group increased(P<0.05).Compared with the model group,the relative expression levels of NRG1 protein in the acupuncture group and the NRG1 group increased(P<0.05);compared with the model group,the rela-tive expression levels of ErbB4 protein in the acupuncture group increased(P<0.05).Conclusion Acupuncture can improve the behavioral activities of ASD,especially the core symptom of social interaction disorder,which may be related to the regulation of the NRG1/ErbB4 signaling pathway and the promotion of NRG1 and ErbB4 expression.关键词
孤独症谱系障碍/NRG1/ErbB4信号通路/针刺/社会交往障碍/行为学Key words
autism spectrum disorder/NRG1/ErbB4 signaling pathway/acupuncture/impaired social interaction/behavior引用本文复制引用
庄婉玉,洪钰,张颖莹,陈思洁,黄龙生,康洁..基于NRG1/ErbB4信号通路探讨针刺对孤独症谱系障碍行为学的影响[J].康复学报,2025,35(1):44-50,7.基金项目
国家自然科学基金项目(81804173) （81804173）
福建省自然科学基金项目(2022J01363) （2022J01363）
福建省科技创新联合资金项目(2020Y9145) （2020Y9145）
