康复学报2025，Vol.35Issue(1)：51-58,8.DOI:10.3724/SP.J.1329.2025.01007
基于Wnt/β-catenin信号通路探讨游泳抑制结直肠癌进展的作用机制
Mechanism of Swimming-Induced Inhibition of Colorectal Cancer Progression via the Wnt/β-catenin Signaling Pathway
摘要
Abstract
Objective To explore the mechanism by which swimming inhibits the progression of colorectal cancer(CRC)in mice through the Wnt/β-catenin signaling pathway.Methods In vitro experiments were conducted where CT26 cells in good growth condition were randomly divided into negative control group(si NC group)and β-catenin knockdown group(si β-catenin group).Af-ter knockdown of the β-catenin gene by siRNA technology,the proliferation of CT26 cells was observed under a microscope,and the cells were counted.Western blot method was used to detect the expression levels of downstream related factors β-catenin,c-Myc,Cyclin D1,and Axin2 in the Wnt/β-catenin signaling pathway.In vivo experiments,sixteen healthy male 8-week-old BALB/C mice were randomly divided into control group and swimming group with 8 mice in each group.The control group was placed in a dry container,while the swimming group underwent swimming training in a pool,six times a week,30 minutes each time,for a total of 3 weeks.The mice were then sacrificed and their body weight was measured.The levels of alanine aminotransferase(ALT),aspar-tate aminotransferase(AST),creatinine(Cr),and blood urea nitrogen(BUN)in the serum were detected using corresponding assay kits;Tumor volumes were determined by electronic vernier calipers,and the tumor mass was weighed and photographed.Immuno-histochemistry(IHC)was used to detect the expression of proliferating cell nuclear antigen Ki-67 in tumor tissues.Western blot was used to detect the relative expression levels of total protein phospho-β-catenin and nuclear β-catenin in tumor tissues.Results Compared with the si NC group,the cell number in the si β-catenin group was significantly reduced(P<0.05);Compared with the si NC group,the protein expression levels of β-catenin,c-Myc,Cyclin D1,and Axin2 in the si β-catenin group significantly decreased(P<0.05).Compared with the control group,the swimming group showed no statistically significant difference in body weight(P>0.05).Compared with the control group,the swimming group showed no statistically significant difference in the levels of ALT,AST,Cr and BUN in the serum(P>0.05).Compared with the control group,the tumor volume was significantly reduced(P<0.05),and tumor mass decreased in the swimming group(P<0.05).Compared with the control group,the expression of Ki-67 in the tumor tissues of the swimming group significantly decreased(P<0.05);compared with the control group,the total protein level of p-β-catenin significantly increased(P<0.05),and the expression level of nuclear β-catenin in the swimming group decreased significant-ly(P<0.05).Conclusion This study indicates that swimming can inhibits the progression of colorectal tumors in mice by downreg-ulating the Wnt/β-catenin signaling pathway,suggesting that swimming may serve as a potential adjuvant therapy for the recovery of CRC patients mediated by the nuclear translocation of β-catenin.关键词
结直肠癌/Wnt/β-catenin信号通路/结肠腺癌细胞/游泳/Ki-67Key words
colorectal cancer/Wnt/β-catenin signaling pathway/colon adenocarcinoma cell/swim/Ki-67引用本文复制引用
林铭,马恩,袁梦,沃达,朱伟东,方霞,任丹妮..基于Wnt/β-catenin信号通路探讨游泳抑制结直肠癌进展的作用机制[J].康复学报,2025,35(1):51-58,8.基金项目
国家自然科学基金项目(82103412) （82103412）
福建中医药大学青年科研拔尖人才项目(XQB202201) （XQB202201）
福建中医药大学高层次人才科研项目(X2021001-人才) （X2021001-人才）
