辐射防护2025，Vol.45Issue(2)：182-188,7.
耐辐射球菌PprI基因腺病毒载体的构建及生物信息学分析
Components and bioinformatics analysis of adenovirus vector of the PprI gene of deinococcus radiodurans
王志鹏 1赵璐 2李建华 1刘欢 1陈建华1
作者信息
- 1. 中国辐射防护研究院,太原 030006
- 2. 山西省人民医院实验动物中心,太原 030012
- 折叠
摘要
Abstract
PprI gene has obvious prevention and control effect on acute radiation damage caused by neutron and gamma rays in mammals.In this study,the PprI gene was directed cloned into the shuttle plasmid pADV-mCMV-MCS-3xFLAG using the non-replicating adenovirus vector system AdMax packaging system,and then co-transfected into HEK293 cells with the adenovirus skeleton plasmid for homologous recombination.The recombinant adenovirus pAdMax-PprI was obtained and identified.The PprI protein was bioinformatics analyzed.PCR results of recombinant adenovirus pAdMax-PprI showed that the inserted fragment was consistent with the size of the PprI gene fragment,and sequencing showed that the inserted fragment was consistent with the PprI gene sequence.Western blot results showed that PprI protein was immunogenic with a molecular weight of about 45 kDa.Bioinformatics analysis showed that PprI gene encodes a hydrophilic protein composed of 163 amino acid residues,which exists in cell membrane,has no signal peptide and transmembrane structure,and has 5 antigenic epitopes.The secondary structure includes three forms of random curling,α-helix and β-folding.Random curl(46.73%)and α helix(28.83%)were the main structures.The prediction results of tertiary structure were consistent with those of secondary structure.The results laid a foundation for further studies on the function of PprI and its radiation resistance.关键词
腺病毒/耐辐射球菌/PprI基因Key words
adenovirus/radiococcus durans/PprI gene分类
生物科学引用本文复制引用
王志鹏,赵璐,李建华,刘欢,陈建华..耐辐射球菌PprI基因腺病毒载体的构建及生物信息学分析[J].辐射防护,2025,45(2):182-188,7.
