分析测试学报2025，Vol.44Issue(3)：393-401,9.DOI:10.12452/j.fxcsxb.240620153
基于还原氧化石墨烯/金纳米粒子/多壁碳纳米管复合材料构建酶生物燃料电池自供能葡萄糖生物传感器研究
An Enzymatic Biofuel Cells-based Self-powered Glucose Biosensor Based on Reduced Graphene Oxide/Gold Nanoparticles/Multi-walled Carbon Nanotubes Nanocomposite
摘要
Abstract
Enzymatic biofuel cells(EBFC)-based self-powered sensing device has the advantages of simple structure,easy miniaturization,and no need for external power supply.It exhibits potential application prospects in clinical diagnosis,environmental monitoring,biosensing and other fields.Reduced graphene oxide/Aurum nanoparticles/multi walled carbon nanotubes(rGO/AuNPs/MWCNTs)nanocomposite was used as electrode substrate materials.The bioanode and cathode of enzymatic bio-fuel cells were prepared via immobilization of glucose oxidase(GOx)and electroposition growth of manganese dioxide(MnO2)on the surface of rGO/AuNPs/MWCNTs modified electrodes,respective-ly.An enzymatic biofuel cells-based self-powered glucose biosensor(EBFC-SPGB)was constructed by combining as-prepared bioanode and cathode in supporting electrolyte at room temperature.In the presence of target glucose,the GOx fixed on the surface of the bioanode promotes an enzymatic reac-tion.The electrons generated by catalyzing glucose transferred to the cathode through an external cir-cuit,resulting in a reduction reaction of MnO2 on the cathode surface and generating an electrochemi-cal response signal.Due to the excellent conductivity,biocompatibility and large specific surface ar-ea of rGO/AuNPs/MWCNTs nanocomposite,the synergistic effect of nanocomposite can significantly increase loading amount of GOx and effectively promote electron transfer on the electrode surface.The maximum power output signal of the constructed EBFC-SPGB shows a good linear relationship with glucose concentration in the range of 1-30 mmol/L,with a detection limit(S/N=3)of 0.3 mmol/L,which can be applied to analysis of glucose concentration in human serum samples.关键词
葡萄糖/纳米复合材料/酶生物燃料电池/自供能葡萄糖生物传感器Key words
glucose/nanocomposite/enzymatic biofuel cells/self-powered glucose biosensor分类
化学引用本文复制引用
易锦飞,尹学虎,李蓉,马丽红,张艳丽,王红斌,杨文荣,庞鹏飞..基于还原氧化石墨烯/金纳米粒子/多壁碳纳米管复合材料构建酶生物燃料电池自供能葡萄糖生物传感器研究[J].分析测试学报,2025,44(3):393-401,9.基金项目
国家自然科学基金资助项目(21665027,21565031) （21665027,21565031）
云南省科技厅应用基础研究项目(202001AT070012) （202001AT070012）
云南民族大学研究生科研创新基金项目(2024SKY135) （2024SKY135）
