分析测试学报2025，Vol.44Issue(3)：438-444,7.DOI:10.12452/j.fxcsxb.240710207
基于FSPE/UPLC-MS/MS-QTRAP快速测定奶粉中3种胃动力药物
Rapid Determination of Three Gastric Motility Drugs in Milk Powder by FSPE/UPLC-MS/MS-QTRAP
摘要
Abstract
A rapid and accurate method for the simultaneously determination of 3 gastric motility drugs(domperidone,mosapride and cisapride)in milk powder was developed in this study using fil-ter-through solid phase extraction(FSPE)/ultra high performance liquid chromatography-quadrupole-linear ion trap mass spectrometry(UPLC-MS/MS-QTRAP).Samples were dissolved in warm water at 45℃,extracted using 10 mL acetonitrile,centrifuged and purified by Oasis PRiME HLB solid phase extraction column for reducing impurity interference in samples.The separation of 3 target com-pounds was performed on an Agilent Poroshell 120 EC-C18 column(100 mm×3.0 mm,2.7 µm)us-ing gradient elution of methanol and 20 mmol/L ammonium acetate aqueous solution as mobile phas-es.Analytes were detected in positive electrospray ionization(ESI+)with multiple reaction monitoring(MRM)mode scanning,and quantitatively analyzed using the internal standard method.The 3 kinds of gastric motility drugs showed good linear relationship in the range of 0.05-5.0 µg/L with cor-relation coefficients(r2)not less than 0.999 4.The detection limits reached 0.07 µg/kg,and the quantitation limits reached 0.2 µg/kg.The average recoveries of actual blank spiked sample ranged from 93.2%to 105%,and the relative standard deviations(RSDs)ranged from 1.3%to 10%.This method has higher analysis efficiency,higher sensitivity and accuracy,and is suitable for rapid de-tection of 3 gastric motility drugs in milk powder.关键词
通过式固相萃取/超高效液相色谱-三重四极杆-线性离子阱质谱/奶粉/胃动力药物Key words
filter-through solid phase extraction(FSPE)/ultra high performance liquid chromatog-raphy-quadrupole-linear ion trap mass spectrometry(UPLC-MS/MS-QTRAP)/milk powder/gas-tric motility drugs分类
化学引用本文复制引用
张宪臣,张朋杰,胡仪光,王勇,华洪波..基于FSPE/UPLC-MS/MS-QTRAP快速测定奶粉中3种胃动力药物[J].分析测试学报,2025,44(3):438-444,7.基金项目
拱北海关科技项目(2023GK016) （2023GK016）
