分析测试学报2025，Vol.44Issue(3)：445-453,9.DOI:10.12452/j.fxcsxb.240602104
基于F68-Ce-Cu过氧化酶活性调控检测类烟产品中尼古丁
Detection of Nicotine in Cigarette-like Products Based on the Regulation of Peroxidase-like Activity of F68-Ce-Cu
摘要
Abstract
Cerium and copper doped nanozyme(F68-Ce-Cu)with peroxidase-like activity was pre-pared for the colorimetric analysis of nicotine in"tea tobacco"and"flower tobacco".In the pres-ence of H2 O2,F68-Ce-Cu could catalyze the conversion of colorless 3,3',5,5'-tetramethylbenzi-dine(TMB)into blue oxidized TMB(oxTMB).It had been found that nicotine has a significant sensi-tizing effect on the peroxidase-like activity of F68-Ce-Cu without interference from other possible components in cigarette-like products.The results of the Michaelis-Menten kinetics study showed that compared to the Ce(Km=2.04 mmol/L)or Cu(Km=9.46 mmol/L)based-nanozyme alone,the Km of F68-Ce-Cu was significantly reduced(Km=1.51 mmol/L),and the Km of"F68-Ce-Cu+nicotine"system was 0.25 mmol/L,which was 6 times more strongly bound to the substrate after the addition of natural nicotine.The results of Zeta potential determination showed that there was a strong adsorp-tion between F68-Ce-Cu and nicotine,and it could promote the production of more·OH and O2·-,which significantly increased the peroxidase-like activity.In the range of 0.017-0.52 mg/mL,nico-tine concentration showed a good linear relationship with absorbance at 654 nm,and the detection limit reached 0.008 mg/mL.In the actual cigarette-like products,the recovery of the method was in the range of 90.9%-103%,and the relative standard deviation was in the range of 2.1%-4.6%,which was almost consistent with the detection results of high performance liquid chromatography,in-dicating that the method was stable and accurate.关键词
尼古丁/类烟产品/纳米酶/过氧化物酶/比色检测Key words
nicotine/cigarette-like products/nanozyme/peroxidase-like activity/colorimetric detection分类
化学引用本文复制引用
陈丹,杨德志,杨亚玲,王春琼,张轲,荣先芳,龙杰,李海燕,蔡洁云,陈志超,崔一凡..基于F68-Ce-Cu过氧化酶活性调控检测类烟产品中尼古丁[J].分析测试学报,2025,44(3):445-453,9.基金项目
云南省烟草质量监督检测站一般项目(20220001,20240002) （20220001,20240002）
中国烟草总公司云南省公司科技计划重点项目(2023530000241027) （2023530000241027）
