分析测试学报2025，Vol.44Issue(3)：493-499,7.DOI:10.12452/j.fxcsxb.240623167
超高效液相色谱-串联质谱法同时测定酵素梅中酚丁及其9种酯类衍生物和5种类似物
Simultaneous Determination of Oxyphenisatin,Its 9 Ester Derivatives and 5 Analogues in Ferment Plum Using Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
In this study,a novel method for the simultaneous detection and quantification of oxy-phenisatin,its nine ester derivatives(oxyphenisatine diacetate,oxyphenisatine dipropionate,dicy-clopropafenedine,oxyphenisatine dibutyrate,oxyphenisatine divalerate,oxyphenisatine dihexyles-ter,dicyclohexylphenol butyl,oxyphenisatine dienanthate and oxyphenisatine dioctanoate),and five analogues(bisoxatin,4-chloro-oxyphenisatine,bisoxatin acetate,4-chloro-oxyphenisatine diac-etate and triacetyldiphenolisatin)in ferment plum using ultra performance liquid chromatography-tan-dem mass spectrometry(UPLC-MS/MS).The sample was ultrasonically extracted with methanol,volume fixed and centrifuged.The separation was performed by Waters Acquity BEH C18 column(50 mm×2.1 mm,1.7 µm)by gradient elution with 5 mmol/L ammonium acetate aqueous solution and acetonitrile as mobile phases.The compounds were step scanned using an electrospray ionization source in the positive and multiple reaction monitoring(MRM)modes,and analyzed by matrix matched external standard method.The results showed that 15 compounds achieved great linearity in the linear ranges,with a correlation coefficients(r2)not less than 0.998 1.The limits of detection(LODs)of 15 compounds ranged from 0.005 to 0.025 mg/kg,and the limits of quantification(LO-Qs)ranged from 0.015 to 0.075 mg/kg.The average recoveries ranged from 72.8%to 114%,while the relative standard deviations(RSDs)were between 1.1%to 9.0%.The method was successfully ap-plied to the determination of 10 fermenter plum samples obtained from different Taobao shops.A total of 9 illegal adulterants were detected,namely oxyphenisatin,oxyphenisatinedipropionate,oxy-phenisatine dibutyrate,oxyphenisatinedivalerate,oxyphenisatine dihexylester,dicyclohexylphenol butyl,bisoxatin,4-chloro-oxyphenisatine,4-chloro-oxyphenisatine diacetate.The contents ranged from 0.1 to 851.9 mg/kg.The method is accurate,sensitive,rapid,and it is suitable for detection of the 15 illegal adulterants in ferment plum.关键词
酚丁/酯类衍生物/类似物/超高效液相色谱-串联质谱/酵素梅Key words
oxyphenisatin/ester derivatives/analogues/ultra performance liquid chromatogra-phy-tandem mass spectrometry(UPLC-MS/MS)/ferment plum分类
化学化工引用本文复制引用
夏宝林,扈战强,张亚清,殷晶晶,汪仕韬,杨娜,张维益..超高效液相色谱-串联质谱法同时测定酵素梅中酚丁及其9种酯类衍生物和5种类似物[J].分析测试学报,2025,44(3):493-499,7.基金项目
江阴市社会发展科技示范项目(JY0603A011014230014PB,JY0603A011014230027PB) （JY0603A011014230014PB,JY0603A011014230027PB）
上海中侨职业技术大学校级课题(ZQZR202439) （ZQZR202439）
