分析测试学报2025，Vol.44Issue(3)：521-526,6.DOI:10.12452/j.fxcsxb.240621160
双光子显微镜二次谐波成像条件探索及其应用
Exploration and Application of Second Harmonic Imaging Using Two-photon Microscope
摘要
Abstract
Second harmonic generation(SHG)has been applied widely for label-free,low light toxic-ity and sensitive to structure.The conditions of SHG imaging on a two-photon microscope were ex-plored by using in vivo mouse skin.Glycerol was used as a clearing agent.The SHG signal intensity and the imaging depth of the skin were statistically evaluated after glycerol and water treatments.The SHG signal intensity and imaging depth showed significant differences between the glycerol-treated and water-treated groups(* p≤0.05),suggesting that glycerol serves as an efficient clearing agent for in vivo skin studies.Through an exploration of laser performance(output power),the autofluores-cence properties of biological tissues,and imaging depth,the optimal excitation wavelength for SHG imaging with a two-photon microscope was 900 nm.With the application of glycerol and the optimal excitation wavelength,SHG signals from tumor skin can be collected,allowing the study of effects of tumors on collagen fiber in vivo.The results show that the two-photon microscope can effectively per-form SHG imaging and suitable for observation and visualization research of the collagen fiber struc-ture within biological tissues.关键词
双光子显微镜/二次谐波产生/肿瘤/胶原Key words
two-photon microscope/second-harmonic generation/tumor/collagen分类
化学化工引用本文复制引用
韩琴,吴航军,武灵云,宋丹丹..双光子显微镜二次谐波成像条件探索及其应用[J].分析测试学报,2025,44(3):521-526,6.基金项目
浙江大学实验技术研究项目一般项目(SYBJS202208) （SYBJS202208）
浙江大学实验技术研究项目重点项目(SZD202102) （SZD202102）
