分子影像学杂志2025，Vol.48Issue(2)：131-137,7.DOI:10.12122/j.issn.1674-4500.2025.02.01
[18F]JR-1001对恐惧记忆疾病的诊断价值
Diagnosis value of[18 F]JR-1001 in the fear memory disorders
摘要
Abstract
Objective To investigate the changes in the expression of cannabinoid type 1 receptor(CB1R)in the brains of fear memory model mice using[2-(2-chlorophenyl)-3-{4-[(2-18F-fluoroethyl)oxy]phenyl}-5,6,7,8-tetrahydrooxepino[3,2-c]pyrazol-8-yl]amino]methanoic acid methyl ester([18F]JR-1001)as a tracer through positron emission tomography(PET).Methods[18F]JR-1001 was automatically prepared using a fused automatic synthesizer and subjected to quality control tests.Fear memory model mice were established according to established modeling protocols.In vitro stability tests were conducted,and quantitative analysis of brain region uptake was performed using MicroPET/CT imaging and a gamma counter.Results The synthesized compound was confirmed to be the target product and met medical usage requirements.Successful modeling was confirmed based on the testing methods for fear memory model mice.In vitro stability data indicated that[18F]JR-1001 expressed stably in vivo and suggested hepatic metabolism.MicroPET/CT imaging and gamma counter data revealed significant differences in the uptake of[18F]JR-1001 between experimental groups(fear memory model mice)and control groups(normal C57 mice)in various brain regions(P<0.05).Notably,the uptake values of[18F]JR-1001 in multiple brain regions of fear memory model mice were significantly higher than those in normal C57 mice.Conclusion The synthesized product was identified as[18F]JR-1001,and its quality control and in vitro stability tests met the requirements for medical research.Furthermore,data obtained from MicroPET/CT imaging and gamma counters have provided new insights and methods for the early diagnosis and treatment of fear memory disorders using PET technology targeting CB1R.Additionally,the findings of this study not only validate the correlation between fear memory and CB1R expression but also uncover the pivotal role of CB1R in the formation of fear memory,offering significant references for research in related fields.关键词
正电子放射计算机断层成像/大麻素1型受体/放射性示踪剂/恐惧记忆疾病/早期诊断Key words
positron emission tomography/cannabinoid type 1 receptor/radiotracer/fear memory disease/early diagnosis引用本文复制引用
毛迪龙,豆晓锋,钟燕,金晨涛,张宏,魏毅,缪立威,陈俊威,曹星海,和庆钢,于聪聪,王菁,曹淑霞..[18F]JR-1001对恐惧记忆疾病的诊断价值[J].分子影像学杂志,2025,48(2):131-137,7.基金项目
国家自然科学基金委重大仪器专项(32027802) （32027802）
浙江省"领雁"研发攻关计划基金项目(2022C03071,2024C03100)Supported by The Major Instrument Special Project of the National Natural Science Foundation of China(32027802) （2022C03071,2024C03100）
