肝胆胰外科杂志2025，Vol.37Issue(2)：93-99,7.DOI:10.11952/j.issn.1007-1954.2025.02.004
胆囊切除术与胆管结石的关联:一项孟德尔随机化研究
Associations of cholecystectomy with calculus of bile duct:a Mendelian randomization study
曹佳辉 1胡彦建 2孙思宇 3胡彦华 3贺旺 4姜博3
作者信息
- 1. 哈尔滨医科大学第二临床医学院,黑龙江 哈尔滨 150081
- 2. 哈尔滨市第一医院消化科,黑龙江 哈尔滨 150010
- 3. 哈尔滨医科大学附属第二医院 普外科,黑龙江 哈尔滨 150086
- 4. 哈尔滨医科大学附属第二医院 急诊外科,黑龙江 哈尔滨 150086
- 折叠
摘要
Abstract
Objective To explore the causal relationship between cholecystectomy and calculus of bile duct by using Mendelian randomization analysis.Methods Genetic variations(P<1×10-5)significantly related to cholecystectomy and calculus of bile duct were obtained from the Public Genome-Wide Association Study(GWAS)database.Cholecystectomy was set as the exposure factor,and calculus of bile duct as the outcome factor.The linkage disequilibrium parameters were set as r2<0.01 and genetic distance was kb>10 000 to exclude linkage disequilibrium.LDtrait was used to manually remove confounding factors.SNPs with F statistics>10 were included in the analysis and selected as instrumental variables(IVs)for the exposure factor.Mendelian randomization analysis was performed by using inverse variance weighting(IVW),MR-Egger,weighted median,simple mode,and weighted mode analysis methods,to evaluate the causal relationship between cholecystectomy and calculus of bile duct.Cochran's Q test was used for heterogeneity analysis of IVW and MR-Egger,and MR-Egger intercept for horizontal pleiotropy.The stability of the results was verified by using the leave-one-out method.Results Screening and including 11 instrumental variables(IVs)related to the exposure.The IVW result showed a causal relationship between cholecystectomy and calculus of bile duct(OR=1.179,95%CI 1.123 to 1.238,P=3.786×10-11).The MR analysis results of the MR-Egger,weighted median method,simple mode method,and weighted mode method(OR>1)all supported a positive correlation between cholecystectomy and calculus of bile duct.The Cochran's Q test of IVW and MR-Egger and the MR-Egger intercept term showed no statistical heterogeneity(P<0.05)or horizontal pleiotropy(P<0.05)among IVs highly related to cholecystectomy.The leave-one-out method showed stable analysis results.Conclusion Our study suggests that cholecystectomy may increase the risk of calculus of bile duct,but further clinical equivalence studies are needed to confirm this finding.关键词
胆囊切除术/胆管结石/孟德尔随机化/逆方差加权法/因果关系Key words
cholecystectomy/calculus of bile duct/Mendelian randomization/inverse variance weighting method/causal relationship分类
临床医学引用本文复制引用
曹佳辉,胡彦建,孙思宇,胡彦华,贺旺,姜博..胆囊切除术与胆管结石的关联:一项孟德尔随机化研究[J].肝胆胰外科杂志,2025,37(2):93-99,7.
