西部中医药2025，Vol.38Issue(2)：1-5,5.DOI:10.12174/j.issn.2096-9600.2025.02.01
四妙凉血颗粒5种成分含量测定及对急性痛风性关节炎大鼠的抗炎作用研究
Determination of the Contents of Five Components in Simiao Liangxue Granules and Its Anti-inflammatory Effects on Acute Gouty Arthritis Rats
摘要
Abstract
Objective:To establish the determination methods for the contents of five components in Simiao Liangxue granules and to discuss the anti-inflammatory mechanism of the medicine on rats with acute gouty arthritis(AGA).Methods:HPLC was used to detect the contents of five components in the medicine;AGA rat models were prepared by injecting sodium urate suspension into foot and ankle,the swelling degree of ankle joint was detected by volume method;ELISA method was adopted to measure the contents of IL-1β,IL-6 and TNF-α in the serum and joint fluid;immunohistochemical and image analysis methods were utilized to observe the expressions of TNF-α protein in cartilage tissue of ankle.Results:The results of methodological examination showed that all the indicators conformed to the regulations,average recovery rates of astilbin,berberine hydrochloride,kaempferol,baicalein and scutellarin ranged between 97.14%and 102.67%in the samples of the medicine,RSD values were smaller than 3.0%.After the injection of sodium urate suspension,compared with blank control group,more significant swelling of ankle joint could be seen,the levels of IL-1β,IL-6 and TNF-α in the joint fluid,the expressions of IL-6 and TNF-α in the serum were elevated in the model group at different time points(P<0.05);small amounts of TNF-α protein expressions could been found in the blank control group.Compared with the model group,the swelling of ankle joints in the positive group(colchicin,0.65 mg/kg),high,moderate and low doses groups of Simiao Liangxue granules(6,3 and 1.5 g/kg)was alleviated from the third to the fifth day,the contents of IL-1β,IL-6 and TNF-α in joint fluid,the levels of IL-6 and TNF-α in the serum were reduced(P<0.05);TNF-α protein integral absorbance was lowered in the low,moderate and high doses groups of the medicine(P<0.05).Compared with the positive group,the swelling of ankle joints,the contents of IL-1β,IL-6 and TNF-α in joint fluid,the levels of IL-6 and TNF-α in the serum,as well as TNF-α protein integral absorbance in the joint tissue were changed insignificantly,and the difference had no statistical meaning(P>0.05).Conclusion:The determination method for the contents of the granules established in the study,exclusive,reproducible and accurate,could be used for the content determination of astilbin,berberine hydrochloride,kaemp-ferol,baicalein and scutellarin in the samples of the granules;the mechanism of treating AGA with the medicine may be related to the reduction in the levels of local inflammation factors IL-1β,IL-6 and TNF-α in the joints.关键词
急性痛风性关节炎/四妙凉血颗粒/白细胞介素1β/白细胞介素6/肿瘤坏死因子αKey words
acute gouty arthritis/Simiao Liangxue granules/IL-1β/IL-6/TNF-α分类
医药卫生引用本文复制引用
唐春丽,农必华,冯茵怡,林忆龙,魏江存,罗远..四妙凉血颗粒5种成分含量测定及对急性痛风性关节炎大鼠的抗炎作用研究[J].西部中医药,2025,38(2):1-5,5.基金项目
国家中医药管理局"十二五"中医药重点学科(民族药学)壮药学资质项目 （民族药学）
全国中药特色技术传承人才培训项目(20184828005) （20184828005）
广西自然科学基金项目(2020GXNSFAA259059) （2020GXNSFAA259059）
广西中医药大学高层次人才培育创新团队资助项目(2022A008). （2022A008）
