西部中医药2025，Vol.38Issue(2)：6-10,5.DOI:10.12174/j.issn.2096-9600.2025.02.02
薯蓣皂苷元对非酒精性脂肪性肝病小鼠脂质代谢的影响
Impacts of Diosgenin on Lipid Metabolism in Non-alcoholic Fatty Liver Disease Mice
摘要
Abstract
Objective:To survey the influence of diosgenin on the levels of TG and TC,lipid deposition in liver tissue of the mice with non-alcoholic fatty liver disease(NAFLD)induced by high fat diet.Methods:Forty male ICR mice were allocated to the control group,the model group,high and low dosage groups of diosgenin,and fenofibrate group with eight in each group.The control group was fed with common fodder,the model group was given high-fat feed with 45%fat for energy;diosgenin mixed forage,that is,each kilogram of high-fat feed contained 10 g and 5 g of diosgenin respectively,was administered to high and low dosage groups of diosgenin respectively;fenofibrate-mixed fodder,that is,each kilogram of high-fat feed included fenofibrate 200 mg,was given to fenofibrate group,all the groups accepted 16 consecutive weeks of the treatment.HE staining was applied to observe the pathomorphology of liver tissue;oil red O(ORO)staining was utilized to observe the fat deposition in the liver;to detect the expressions of serum TG and TC.Results:HE staining results displayed that the degrees of adipose degeneration of the model group were severer than these of the control group,intracytoplasmic vacuola-tion was reduced in high and low dosage groups of diosgenin,and fenofibrate group.ORO staining showed that:fat contents of the liver tissue of the model group were higher than these of the control group,while fat contents in hepatic cell were lowered in high and low dosage groups of diosgenin,and fenofibrate group.Compared with the control group,the levels of TG and TC were elevated in the model group(P<0.01);Compared with the model group,the levels of TG and TC were lowered in high and low dosage groups of diosgenin,and fenofibrate group(P<0.01);no statistical difference was found in the levels of TG and TC when high and low dosage groups of diosgenin were compared with fenofibrate group(P>0.05).Conclusion:Diosgenin could reduce adipose degenera-tion in hepatic cells and fat contents in NAFLD mice models induced by high-fat food,and the expressions of TG and TC.关键词
脂肪性肝病,非酒精性/脂质代谢/薯蓣皂苷元/小鼠Key words
fatty liver disease,non-alcoholic/lipid metabolism/diosgenin/mice分类
医药卫生引用本文复制引用
梁淑芳,黄国开,聂姝常,程彬彬,刘群..薯蓣皂苷元对非酒精性脂肪性肝病小鼠脂质代谢的影响[J].西部中医药,2025,38(2):6-10,5.基金项目
国家自然科学基金面上项目(81774077) （81774077）
国家自然科学基金青年基金(82405513) （82405513）
海军军医大学第一附属医院基础研究专项(2023QD21) （2023QD21）
海军军医大学第一附属医院青年启动基金项目(2019QNA06). （2019QNA06）
