甘肃中医药大学学报2025，Vol.42Issue(1)：1-7,7.DOI:10.16841/j.issn1003-8450.2025.01.01
红景天苷对低氧诱导大鼠心肌细胞凋亡及氧化应激损伤、炎症反应的影响
Effects of salidroside on apoptosis,oxidative stress injury and inflammation of cardiomyocytes induced by hypoxia in rats
摘要
Abstract
Objective To investigate the effects of salidroside(SAL)on hypoxia-induced apoptosis,oxidative stress injury and inflammatory response in rat cardiomyocytes.Methods H9C2 rat cardiomyocytes were used.The experiment was divided into normoxia group(21％O2)and hypoxia group(2％O2).Cardiomyocytes in the hypoxia group were first treated with hypoxia for 24 hours in a dedicated hypoxia incubator,then drugs were added,and then they were treated under hypoxia for another 24 hours.Cell viability was detected by CCK-8 kit,the degree of oxida-tive stress injury was observed by detecting lactate dehydrogenase(LDH)activity,the levels of inflammatory factors interleukin(IL)-1β and IL-6 were determined by enzyme-linked immunosorbent assay(ELISA),and the relative ex-pression levels of apoptosis-related genes hypoxia-inducible factor 1α(HIF-1α)and Caspase3 mRNA were detected by real-time quantitative reverse transcription polymerase chain reaction(RT-qPCR).Results Compared with the normoxia group,after hypoxia induction,the cell viability of rat cardiomyocytes decreased significantly,the intracel-lular LDH activity and the levels of inflammatory factors IL-1β and IL-6 increased significantly,and the expression of Caspase3 and HIF-1α mRNA was significantly upregulated,with statistically significant differences(P＜0.05 or P＜0.01 or P＜0.001);20 μmol/L SAL could significantly increase the viability of cardiomyocytes,reduce LDH ac-tivity and the levels of IL-1β and IL-6,and reduce the expression of Caspase3 and HIF-1α mRNA,with statistically significant differences(P＜0.05 or P＜0.01 or P＜0.001).Conclusion SAL can effectively alleviate hypoxia-induced oxidative stress injury and inflammatory response in rat cardiomyocytes and inhibit cardiomyocyte apoptosis.关键词
红景天苷/心肌细胞/低氧/心血管疾病/氧化应激损伤/炎症反应/细胞凋亡/大鼠/实验研究Key words
salidroside/cardiomyocytes/hypoxia/cardiovascular diseases/oxidative stress injury/inflammatory response/apoptosis/rats/experimental study分类
医药卫生引用本文复制引用
向权,杨榛,施树德,张凌云,韦文静,赵生国,刘凯..红景天苷对低氧诱导大鼠心肌细胞凋亡及氧化应激损伤、炎症反应的影响[J].甘肃中医药大学学报,2025,42(1):1-7,7.基金项目
国家自然科学基金地区项目(82260869) （82260869）
平凉市崆峒区科技计划项目(重点研发计划)(PL-KT-2024-010). （重点研发计划）
