甘肃中医药大学学报2025，Vol.42Issue(1)：8-13,6.DOI:10.16841/j.issn1003-8450.2025.01.02
家兔不同组织4种RNA相对表达量与死亡时间相关性研究
Study on the correlation between relative expression levels of four types of RNA in different tissues of rabbits and post-mortem interval
摘要
Abstract
Objective To investigate the relationship between post-mortem interval(PMI)and the relative ex-pression levels of four RNAs in liver,brain and spinal cord of rabbits.Methods Twenty-six domestic rabbits were selected and euthanized by drowning.Their carcasses were then placed in an environment with constant temperature(25 ℃)and humidity(50％).RNA was extracted from liver,brain,and spinal cord tissues at eight time points within 0 to 72 hours post-mortem.Real-time fluorescent quantitative polymerase chain reaction(PCR)technology was utilized to detect four types of RNA:ATP6 V0A1 mRNA,DPP4 mRNA,RIMS2 circRNA,and DYM circRNA.SPSS 25.0 statistical software was employed to conduct statistical analysis on the relative expression levels of the tar-get RNAs.Additionally,curve regression analysis was performed between the target RNAs and PMI.Results As PMI increased,the relative expression level of ATP6 V0A1 mRNA in the liver of rabbits was significantly higher than that in the brain and spinal cord.The relative expression level of DPP4 mRNA in the liver began to increase at 8 hours after death,reached a peak at 24 hours,and then decreased sharply,but was still significantly higher than that in the brain and spinal cord.In comparison,the relative expression levels of the two circRNAs,RIMS2 circRNA and DYM circRNA,exhibited a trend of steady and gradual decline in liver,brain,and spinal cord tissues.Among them,the relative expression level of DYM circRNA demonstrated the most stable trend across all three tissue types.Conclusion The expression level of circRNA has a good correlation with PMI.Combined with other molecular markers,it has certain application prospects in forensic PMI estimation and criminal investigation.关键词
死亡时间/环状RNA/信使RNA/相对表达量/实时荧光定量聚合酶链式反应/相关性研究/家兔Key words
postmortem interval/circular RNA/messenger RNA/relative expression level/real-time fluores-cence quantitative polymerase chain reaction/correlation study/rabbits分类
医药卫生引用本文复制引用
吴军,李凯欣,孙兴璐,张舒,刘一君,杨鑫,楚惠媛..家兔不同组织4种RNA相对表达量与死亡时间相关性研究[J].甘肃中医药大学学报,2025,42(1):8-13,6.基金项目
甘肃省自然科学基金项目(20JR5RA621). （20JR5RA621）
