国际口腔医学杂志2025，Vol.52Issue(2)：195-204,10.DOI:10.7518/gjkq.2025008
转铁蛋白修饰的姜黄素脂质体对口腔鳞状细胞癌细胞株HN4增殖抑制机制的研究
Mechanism of transferrin-modified liposomal curcumin on proliferation inhibition of oral squamous cell carcinoma
摘要
Abstract
Objective This study aimed to investigate the effect of transferrin(Tf)-modified curcumin(Cur)-loaded li-posomes(Lips)on the oral squamous cell carcinoma cell line HN4.Methods Cur-Lips and Tf-Cur-Lips were prepared,and their regulatory effects on the internal and external disposal of curcumin were investigated during Cur release in vitro and pharmacokinetics in vivo in rats.Then,HN4 cells were treated with Cur,Cur-Lips,and Tf-Cur-Lips at different con-centrations successively,and cell counting kit-8 was used to detect the effects of different experimental groups on HN4 cell proliferation.Finally,the expression levels of apoptosis-related genes P53 and Fas and the molecular mechanism of transferrin modification on Cur-Lips inhibiting the proliferation and apoptosis of HN4 cells were investigated through real-time fluorescence quantitative polymerase chain reaction.Results Compared with Cur,Cur-Lips considerably pro-longed metabolic time,and transferrin modification further improved the stability of Cur and prolonged the metabolic time of Cur-Lips.Compared with Cur and Cur-Lips,Tf-Cur-Lips markedly enhanced the inhibitory effect on the HN4 cell proliferation and up-regulated the expression of P53 and Fas.Conclusion Tf-Cur-Lips have a stronger inhibito-ry effect on oral squamous cell carcinoma cell HN4 than Cur and Cur-Lips.关键词
姜黄素/脂质体/转铁蛋白/HN4细胞/细胞凋亡Key words
curcumin/liposomes/transferrin/HN4 cells/cell apoptosis分类
医药卫生引用本文复制引用
魏雪琴,巴凯..转铁蛋白修饰的姜黄素脂质体对口腔鳞状细胞癌细胞株HN4增殖抑制机制的研究[J].国际口腔医学杂志,2025,52(2):195-204,10.基金项目
Soft Science Project and Joint Construction Project of Henan Medical Science and Technology Research Plan(LHGJ20220361) （LHGJ20220361）
Henan Province Science and Technology Research Project(232102310491)河南省医学科技攻关计划软科学项目及联合共建项目(LHGJ20220361) （232102310491）
河南省科技攻关项目(232102310491) （232102310491）
