国际口腔医学杂志2025，Vol.52Issue(2)：210-216,7.DOI:10.7518/gjkq.2025029
基于转录组学探讨金担子素A抗白色念珠菌的作用机制
Mechanism of action of aureobasidin A against Candida albicans based on transcriptomics analysis
摘要
Abstract
Objective This study aimed to investigate the antibacterial activity and potential mechanism of aureobasi-dium A(AbA)against Candida albicans(C.albicans),as well as to evaluate its potential as a new antifungal drug in the future.Methods The minimum inhibitory concentration(MIC)of AbA was determined.The effect of MIC on mycelium formation and cell-wall integrity were investigated.Subsequently,transcriptome sequencing was used to investigate the re-sponse mechanism of C.albicans to AbA.Results In vitro results showed that the MIC of AbA was 0.062 5 μg/mL,and it significantly inhibited C.albicans morphogenesis and disrupted cell-wall integrity.Transcriptome sequencing identified 700 differentially expressed genes,including 465 upre-gulated and 235 downregulated genes.Gene ontology enrichment analysis revealed that pathways related to the extracellular region,plasma membrane,fungal cell wall,and redox enzyme activity were the most enriched.Enrichment analysis through the kyoto encyclopedia of genes and genomes indicated that the metabolic pathway was the most significantly enriched pathway.Conclusion AbA exhibited strong inhibitory activity against C.albicans.Its poten-tial antimicrobial mechanisms may include inhibiting hyphal formation,disrupting the plasma membrane,reducing im-mune evasion,and suppressing metabolic activity.Understanding these mechanisms can provide valuable theoretical in-sights into the development of more effective antifungal-treatment strategies.关键词
白色念珠菌/金担子素A/转录组测序/基因组百科全书富集分析/京都基因富集分析Key words
Candida albicans/aureobasidin A/transcriptome sequencing/kyoto encyclopedia of genes and ge-nomes enrichment analysis/gene ontology enrichment analysis分类
口腔医学引用本文复制引用
易嘉欣,廖建春,黄怡,费伟,郭骏..基于转录组学探讨金担子素A抗白色念珠菌的作用机制[J].国际口腔医学杂志,2025,52(2):210-216,7.基金项目
Sichuan Provincial Cadre Health Care Research Topic(2022-209)四川省干部保健科研课题(2022-209) （2022-209）
