广西林业科学2025，Vol.54Issue(1)：1-12,12.DOI:10.19692/j.issn.1006-1126.20250101
马尾松SAUR基因生物信息学分析及其响应侧枝发育的表达
Analysis on Bioinformatics of SAUR Genes in Pinus massoniana and Expressions on Responses to Lateral Branch Developments
摘要
Abstract
In order to provide references for functional analysis on SAUR genes in Pinus massoniana,and ana-lyze their basic characteristics and roles in lateral branch developments of P.massoniana,based on P.massoni-ana transcriptome data obtained by research group at previous stage,SAUR genes in P.massoniana were screened.Bioinformatics of PmSAUR genes were analyzed from phylogenetic,physical and chemical character-istics of proteins,functional structures of proteins,conserved motifs of proteins,and conserved structural do-mains of proteins.Expression patterns of PmSAUR genes on responses to GR24,6-BA,TIS-108 and apical bud removal treatments were analyzed by qRT-PCR.Results showed that a total of 32 SAUR genes(PmSAUR1-PmSAUR32)were identified from P.massoniana transcriptome data.PmSAUR proteins encoded 113-234 ami-no acids.Protein molecular weights ranged from 12 850.98 to 26 563.57 Da.Protein aliphatic indexes ranged from 70.92 to 116.82.87.50% of PmSAUR proteins had alkaline theoretical isoelectric points,87.50% of Pm-SAUR proteins were instable proteins,and all PmSAUR proteins were hydrophilic proteins.All PmSAUR pro-teins had alpha helixes,extended strands,beta turns and random coils,dominating by alpha helixes and random coils.Subcellular localization of PmSAUR proteins were dispersal,mainly distributing in chloroplasts,mito-chondria,cytoplasm and nuclei.Motif1(RFVIPTSYLNHPLFRALLEKA)was highly conservative,which could be important foundation for identifying PmSAUR genes.PmSAUR7,PmSAUR11,PmSAUR21 and PmSAUR32 had tissue expression specificities on 7 d of 6-BA treatment,and all of them were highly expressed in needles and lowly expressed in stems.Through analysis of expression patterns of four PmSAUR genes,it was hypothe-sized that PmSAUR7 might be key gene to effect P.massoniana lateral branch developments.关键词
SAUR基因/生物信息学/侧枝发育/表达模式/马尾松Key words
SAUR gene/bioinformatics/lateral branch development/expression pattern/Pinus massoniana分类
农业科技引用本文复制引用
姚筱敏,胡颖,陈虎,杨章旗..马尾松SAUR基因生物信息学分析及其响应侧枝发育的表达[J].广西林业科学,2025,54(1):1-12,12.基金项目
国家自然科学基金项目(32160382) （32160382）
广西八桂学者专项(2019A26) （2019A26）
广西自然科学基金项目(2019GXNSFBA245064) （2019GXNSFBA245064）
