广西林业科学2025，Vol.54Issue(1)：66-72,7.DOI:10.19692/j.issn.1006-1126.20250109
桉树焦枯病叶片与正常叶片真菌群落结构差异分析
Comparative Analysis on Fungal Community Structures in Leaves Affected by Eucalypt Leaf Blight and Eucalypt Normal Leaves
摘要
Abstract
Eucalypt is important tree species for paper-making and timber in China.Eucalypt leaf blight main-ly caused by fungi belonging to Calonectria is one of significant diseases affecting eucalypt in China,which has resulted in severe economic losses and ecological impacts for eucalypt production regions in China.Taking Eu-calyptus grandis×E.urophylla as materials,differences between communities of phyllosphere fungi in leaves af-fected by leaf blight and normal leaves were compared by sequencing phyllosphere fungi in diseased leaves and normal leaves through Illumina high-throughput sequencing platform and performing biological analysis,which could explore relationships between difference of fungal communities and eucalypt leaf blight,and provide theo-retical basis for development of control techniques for eucalypt leaf blight from perspective of phyllosphere fun-gi.Results showed that fungal communities in diseased leaves comprised 3 phyla,13 classes,27 orders,42 fam-ilies,58 genera,71 species and 136 amplicon sequence variant(ASVs),which had 4 more orders,2 more fami-lies,2 more genera,3 more species and 9 more ASVs than normal leaves.Dominant fungal phyla in diseased and normal leaves were Ascomycota and Basidiomycota.Relative abundance of Basidiomycota extremely signifi-cantly decreased in diseased leaves,while relative abundance of Ascomycota and unclassified_k_Fungi ex-tremely significantly increased in diseased leaves.Main fungal genera in diseased leaves were Calonectria,Cryptococcus and Pallidocercospora.Main fungal genera in normal leaves were Mycotribulus,Pseudocercospora and Taphrina.Calonectria was only found in diseased leaves.Relative abundance of partial fungal communities in eucalypt leaves affected by leaf blight changed significantly.Relative abundance of Calonectria,Zasmidium and Pallidocercospora extremely significantly increased in diseased leaves,among which increase of relative abundance of Zasmidium could serve as biomarker for occurrence of eucalypt leaf blight.关键词
高通量测序/桉树焦枯病/真菌Key words
high-throughput sequencing/eucalypt leaf blight/fungi分类
农业科技引用本文复制引用
伍禄军,于永辉,赵瑛,蒙好生..桉树焦枯病叶片与正常叶片真菌群落结构差异分析[J].广西林业科学,2025,54(1):66-72,7.基金项目
广西林业科技推广示范项目(2024GXLK31) （2024GXLK31）
