南方农业学报2025，Vol.56Issue(1)：180-191,12.DOI:10.3969/j.issn.2095-1191.2025.01.016
垄高与施钾模式对雪茄烟根系形态和钾素代谢特征的影响
Effects of ridge height and potassium application mode on root morphology and potassium metabolism characteristics of cigar tobacco
摘要
Abstract
[Objective]To study the response of cigar tobacco root morphology and potassium metabolism characteris-tics to ridge height and potassium application mode,which could provide theoretical basis for improving the root growth environment of cigar tobacco and enhancing the quality of cigar leaf.[Method]Using the cigar tobacco variety Chuanxue No.1 as the test material,a 2-factor,3-level split-area test of ridge height and split-application of potassium was de-signed,with 3 ridge height levels(35 cm for high ridges,30 cm for medium ridges,and 25 cm for low ridges)as the main zone and 3 modes of potassium application(1 time of potassium application,2 times of potassium application,and 3 times of potassium application)as the subzones,to analyze the root morphology,potassium content,potassium accu-mulation intensity and the expression of key genes of potassium metabolism in the tobacco plants in the different treat-ments.[Result]Root morphology indexes of cigar tobacco showed an overall trend of gradual increase during the growth process.Under the same ridge height,the root morphology indexes were higher in the treatments of 1 and 2 times of potas-sium application than in the treatment of 3 times of potassium application at 40-50 d after transplanting,and the highest in-dexes were found in the treatment of 3 times of potassium application at 50 d after transplanting.Under the same potas-sium application mode,the root morphology indexes were higher in the high and medium ridge height treatments than in the low ridge height treatment.As the growth period progressed,root potassium content showed an overall trend of in-creasing and then decreasing,stem and leaf potassium content gradually decreased,and the potassium accumulation in-tensity of root,stem,leaf and the whole plant increased first and then decreased.Under the same ridge height,the potas-sium content of each organ was higher after 50 d of transplanting in 2 or 3 times of potassium application;the potassium accumulation intensity of each organ was higher in 3 times of potassium application.Under the same potassium applica-tion mode,the potassium content of all organs was higher in the high and medium ridge height treatments at 50 d after transplanting,and the potassium accumulation intensity of root,stem,leaf and whole plant was higher in the high and medium ridge height treatments.The relative expression of inward-flowing potassium ion channel genes NKT1 and NtKC1,potassium transporter protein genes NtTPK1 and NtHAK1 were up-regulated by in 2 and 3 times of potassium ap-plication in high ridges,the relative expression of outward-flowing potassium ion channel gene NTORK1 was low,and the relative expression of potassium transporter protein gene NtKT12 was up-regulated by 3 times of potassium applica-tions in high ridges.[Conclusion]The combination of high and medium ridges(ridge heights 35 and 30 cm)with 3 doses of potassium application(60% potassium base+20% at 30 d after planting+20% at 45 d after planting)is favorable to promote the root system development of cigar tobacco and enhance the capacity of potassium accumulation in tobacco plants.关键词
雪茄烟/垄高/施钾模式/根系形态/钾素代谢/基因表达Key words
cigar tobacco/ridge height/potassium application mode/root morphology/potassium metabolism/gene expression分类
农业科技引用本文复制引用
吴永兵,张瑛,许立南,彭远,何正川,阳苇丽,赵铭钦..垄高与施钾模式对雪茄烟根系形态和钾素代谢特征的影响[J].南方农业学报,2025,56(1):180-191,12.基金项目
中国烟草总公司重大科技项目[110202201036(XJ-07)] （XJ-07）
四川省烟草公司科技项目(SCYC202324) China National Tobacco Corporation Major Science and Technology Project[110202201036(XJ-07)] （SCYC202324）
Sichuan Tobacco Company Science and Technology Project(SCYC202324) （SCYC202324）
