南方农业学报2025，Vol.56Issue(1)：226-235,10.DOI:10.3969/j.issn.2095-1191.2025.01.020
红带滑胸针蓟马(缨翅目:蓟马科)线粒体基因组全序列测定与分析
Whole sequencing and analysis of mitochondrial genome of Selenothrips rubrocinctus(Thysanoptera:Thripidae)
摘要
Abstract
[Objective]To clarify the mitochondrial genome structure of Selenothrips rubrocinctus and analyze its phy-logenetic relationship based on mitochondrial genome sequence,which could provide molecular evidence for identifica-tion and phylogeny of Selenothrips insects.[Method]The mitochondrial genome of Selenothrips rubrocinctus was se-quenced by using Illumina next-generation sequencing,then assembled and annotated.Maximum likelihood(ML)and Bayesian inference(BI)methods were used to construct the phylogenetic tree of 18 Thripidae species and 1 Phlaeothripi-dae species based on the sequences of 13 protein-coding genes(PCGs)of mitochondrial genomes,to analyze the phylo-genetic relationship between S.rubrocinctus and other thrips.[Result]The mitochondrial genome of Selenothrips rubro-cinctus was 14984 bp in length,including 37 genes(13 PCGs,22 tRNA genes,2 rRNA genes)and 1 non-coding region.There were 19 intergenic regions,and the longest intergenic region was 25 bp.There were 10 gene overlapping regions,and the longest overlapping region was 23 bp.The genome had the typical gene composition and order of mitochondrial ge-nome from most Thysanoptera insects,and the AT content was 72.9%,showing obvious AT bias.Among the 13 PCGs,all genes used ATN as the start codon,except for cox1 used TTG as the start codon;and except for nad6 had TAG as the stop codon,2 genes(nad2 and cox2)had incomplete T as the stop codon,another 10 used TAA as the stop codon.Among the 22 tRNA genes,the 17 tRNA genes could form the complete clover-leaf secondary structures,except trnR,trnS2,and trnY lacked TΨC ring,trnS1 and trnV lacked DHU ring.Phylogenetic analysis showed that Selenothrips rubro-cinctus clustered on the same small branch with Rhipiphorothrips cruentatus,and they were closely related.[Conclusion]The mitochondrial genome structure,composition and gene sequence of Selenothrips rubrocinctus are conserved,consis-tent with that of other species of Thripidea.Panchaetothripinae is monophyletic and Selenothrips rubrocinctus is closely re-lated to Rhipiphorothrips cruentatus.关键词
红带滑胸针蓟马/线粒体基因组/系统发育Key words
Selenothrips rubrocinctus/mitochondrial genome/phylogeny分类
植物保护学引用本文复制引用
毛加梅,李佳瑾,王自然,郭俊,张宏瑞..红带滑胸针蓟马(缨翅目:蓟马科)线粒体基因组全序列测定与分析[J].南方农业学报,2025,56(1):226-235,10.基金项目
国家自然科学基金项目(32360129) （32360129）
现代农业产业技术体系建设专项(CARS-26) （CARS-26）
云南省重大科技专项(202102AE0054) （202102AE0054）
云南省院士专家工作站项目(202105AF150071) National Natural Science Foundation of China(32360129) （202105AF150071）
China Agriculture Research System(CARS-26) （CARS-26）
Yunnan Major Science and Technology Special Project(202102AE0054) （202102AE0054）
Yunnan Academician Expert Workstation Project(202105AF150071) （202105AF150071）
