南方农业学报2025，Vol.56Issue(1)：236-249,14.DOI:10.3969/j.issn.2095-1191.2025.01.021
不同发育阶段马铃薯块茎蛾可培养内生细菌多样性及对消化作用的影响
Diversity of culturable endophytic bacteria at different develop-mental stages of Phthorimaea operculella(Zeller)and its effects on digestion
摘要
Abstract
[Objective]To clarify the dynamic changes of culturable endophytic bacteria of Phthorimaea operculella(Zeller)with the growth and development of the host and their effects on feeding and digestive function,which could pro-vide theoretical basis for exploring the interaction between symbiotic bacteria and hosts.[Method]Taking Phthorimaea operculella(Zeller)at different developmental stages as the research object,the culturable endophytic bacteria were iso-lated and cultured in the body(eggs,1st and 2nd instar larvae)or intestine(3rd and 4th instar larvae,male and female pu-pae,male and female adults)of Phthorimaea operculella(Zeller)at different developmental stages by traditional isola-tion and culture technology.The isolated strains were identified by 16S rDNA sequence analysis,through refeeding domi-nant bacteria and resident bacteria,the nutritive indexes of Phthorimaea operculella(Zeller)were detected by gravimetric method,and effects of dominat bacteria and resident bacteria on the feeding and digestion of Phthorimaea operculella(Zeller)larvae were analyzed.[Result]The composition and diversity of culturable endophytic bacteria at different deve-lopmental stages of Phthorimaea operculella(Zeller)were different.The number of culturable endophytic bacteria iso-lated from eggs was the least,and the number of culturable endophytic bacteria isolated from larvae was the most abun-dant.With the increase of age,the number of culturable endophytic bacteria gradually increased,and began to decline af-ter pupation.There were 3 phyla,13 families,17 genera and 30 species of culturable endophytic bacteria in Phthorimaea operculella(Zeller).Among them,there were 2 phyla,3 families,3 genera and 3 species of culturable endophytic bacte-ria in eggs.There were 2 phyla,2 families,2 genera and 3 species;2 phyla,4 families,5 genera and 6 species;3 phyla,9 families,10 genera and 11 species;3 phyla,10 families,10 genera and 11 species;3 phyla,5 families,5 genera and 7 species;3 phyla,8 families,8 genera and 10 species of culturable endophytic bacteria in 1st-4th instar larvae,pupae and adults respectively.The dominant species of endophytic bacteria in Phthorimaea operculella(Zeller)at different develop-mental stages were different.The dominant species of culturable bacteria of the 1st instar larvae was Enterobacter ludwi-gii,while the dominant species of culturable bacteria of eggs,2nd-4th instar larvae,pupae,and adults were Enterococcus mundtii.Enterococcus mundtii was stable at all developmental stages of the Phthorimaea operculella(Zeller),and Serra-tia rubidaea was found in the 3rd and 4th instar larvae,pupae and adults.Enterococcus mundtii,Enterobacter ludwigii and Serratia rubidaea can promote the digestion of Phthorimaea operculella(Zeller).[Conclusion]The species of culturable endophytic bacteria in Phthorimaea operculella(Zeller)are abundant.The composition and structure of culturable endo-phytic bacteria in Phthorimaea operculella(Zeller)at different developmental stages are different.The species of cultura-ble endophytic bacteria in larvae are greatly higher than those in eggs and pupae,but there is no obvious difference in dominant species.The dominant species of endophytic bacteria can promote the growth and development of the host by promoting the digestion of larvae.关键词
马铃薯块茎蛾/内生细菌/可培养细菌/消化作用/营养指标Key words
Phthorimaea operculella(Zeller)/endophytic bacteria/cultural bacteria/digestion function/nutritive index分类
农业科技引用本文复制引用
符乔斯,肖关丽,陈斌,王文倩,杜广祖..不同发育阶段马铃薯块茎蛾可培养内生细菌多样性及对消化作用的影响[J].南方农业学报,2025,56(1):236-249,14.基金项目
国家自然科学基金项目(32360711) （32360711）
云南省教育厅科学研究基金青年人才基础研究专项(2024J0407) National Natural Science Foundation of China(32360711) （2024J0407）
Young Talent Basic Research Project of Scientific Research Fund of Yunnan Education Department(2024J0407) （2024J0407）
