南方农业学报2025，Vol.56Issue(1)：250-260,11.DOI:10.3969/j.issn.2095-1191.2025.01.022
半夏细菌性病害拮抗菌筛选、鉴定及防治效果测定
Screening and identification of antagonistic bacteria against bacterial disease in Pinellia ternata,and determination of its biocontrol efficacy
摘要
Abstract
[Objective]The aim was to explore antagonisitic bacteria against bacterial diseases in Pinellia ternata,which could provide theoretical basis for development and utilization of biocontrol bacterial resources as well as the effi-cient and green control of bacterial disease in P.ternata.[Method]Using 6 strains of pathogenic bacteria of Pinellia ter-nata as target strains,antagonistic bacteria were isolated and screened from the rhizosphere soil of infected Pinellia ter-nata.The strain was identified based on morphological,physiological and biochemical characteristics determination,mo-lecular biology analysis.The in vitro efficacy of the antagonistic bacteria against bacterial diseases caused by Dickeya chrysanthemi,Pseudomonas extremorientalis and Pectobacterium carotovorum in Pinellia ternata,carrots,potatoes and cabbages was determined.The in vivo efficacy of the antagonistic bacteria against bacterial diseases in Pinellia ternata caused by the 3 pathogens was determined by pot experiments.The inhibitory effects of the antagonistic bacteria on 13 pathogenic fungi from traditional Chinese medicinal plants were assessed using the plate confrontation method,clarifying its inhibitory spectrum.[Result]A total of 106 bacterial strains were isolated from the rhizosphere soil of Pinellia ternata,among which strain WBT16 exhibited the best antagonistic effect against 6 pathogenic bacteria causing bacterial diseases in Pinellia ternata.It was identified as Bacillus velezensis.In vitro efficacy tests showed that the strain WBT16 achieved 100% control efficacy against bacterial diseases in Pinellia ternata and carrots caused by Dickeya chrysanthemi,Pseudo-monas extremorientalis and Pectobacterium carotovorum.In vivo efficacy tests demonstrated that strain WBT16 had the highest control efficacy of 76.92% against bacterial diseases in Pinellia ternata caused by Dickeya chrysanthemi,fol-lowed by 69.23% against diseases caused by Pectobacterium carotovorum and 62.50% against those caused by Pseudomo-nas extremorientalis,all exceeding 60.00% .The inhibitory spectrum analysis revealed that antagonistic bacteria WBT16 exhibited fine inhibitory effects against various pathogenic fungi.It showed the best inhibitory effect against Botrytis cine-rea from Fritillaria hupehensis,with an inhibition rate of 86.02%,and relatively high inhibition rates of 80.51% and 79.89% against Sclerotium rolfsii and Sclerotinia sclerotiorum from Pinellia ternata respectively.[Conclusion]The an-tagonistic bacteria WBT16,isolated and screened from the rhizosphere soil of Pinellia ternata,demonstrates effective control against bacterial diseases in Pinellia ternata and exhibits a broad inhibitory spectrum.It holds great development value and application potential for the biological control of bacterial diseases in Pinellia ternata.关键词
半夏/细菌性病害/拮抗菌/防治效果/抑菌谱Key words
Pinellia ternata/bacterial disease/antagonistic bacteria/prevention and control effect/inhibition spec-trum分类
农业科技引用本文复制引用
王帆帆,唐涛,孙光忠,段媛媛,匡辉,王晓月,元维军,郭晓亮,游景茂..半夏细菌性病害拮抗菌筛选、鉴定及防治效果测定[J].南方农业学报,2025,56(1):250-260,11.基金项目
现代农业产业技术体系建设专项(CARS-21) （CARS-21）
湖北省乡村振兴科技示范项目(2024BBB110) （2024BBB110）
湖北省农业科学院青年科技基金项目(2023NKYJJ30) China Agriculture Research System(CARS-21) （2023NKYJJ30）
Hubei Rural Revitalization Science and Tech-nology Demonstration Project(2024BBB110) （2024BBB110）
Science and Technology Fund for Young Scholar of Hubei Academy of Agricultural Sciences(2023NKYJJ30) （2023NKYJJ30）
