工业微生物2025，Vol.55Issue(1)：1-3,3.DOI:10.3969/j.issn.1001-6678.2025.01.001
一株降解聚乳酸微生物的筛选及特性研究
Screening and Characterization of A Poly Lactic Acid Degrading Microorganism
摘要
Abstract
The article screened poly lactic acid(PLA)-degrading microorganisms from the gut of Tenebrio molitor,identified the strains and measured the degradation characteristics of the degrading bacteria.Tenebrio molitor was fed PLA powder for 60 days.Gut extracts of Tenebrio molitor were then inoculated onto solid media with PLA as the sole carbon source for enrichment,isolation and purification of degrading bacteria.Strains were identified via morphological observation and DNA sequencing,and their PLA degradation efficiency was evaluated under various nutrient conditions.A PLA-degrading strain,Fusarium genus LT-1,was isolated and identified as Fusarium spp.It achieved a PLA degradation rate of 10.55％after 40 days of shaking incubation in different media.The results showed that feeding PLA to Tenebrio molitor effectively altered its gut microbiome,and using the inorganic salt culture medium with PLA as the sole carbon source as a screening plate can quickly and efficiently screened PLA-degrading bacteria.The article provides a reference for the biodegradation of PLA plastics.关键词
黄粉虫/聚乳酸/肠道微生物/生物降解Key words
Tenebrio molitor/poly lactic acid/gut microbiome/biodegradation引用本文复制引用
庄昕怡,楼晟豪,李彤,刘超骏,李道霜,冯娟..一株降解聚乳酸微生物的筛选及特性研究[J].工业微生物,2025,55(1):1-3,3.基金项目
2023年浙江省大学生科技创新活动计划暨新苗人才计划(2023R476003) （2023R476003）
2022年浙江省教育厅科研项目(Y202250489) （Y202250489）
台州科技职业学院2022年度校级课题(22NDZ01). （22NDZ01）
