工业微生物2025，Vol.55Issue(1)：81-83,3.DOI:10.3969/j.issn.1001-6678.2025.01.025
消毒时长及间隔对亲和层析填料(MabCapture重组蛋白A)载量的影响研究
Study on the Effect of Disinfection Duration and Interval on the Loading Capacity of Affinity Chromatography Packing Material(MabCapture Recombinant Protein A)
张蕙1
作者信息
- 1. 江苏泰州迈博太科药业有限公司,江苏泰州 225300
- 折叠
摘要
Abstract
By producing the same column height and reducing the column diameter,the paper used the linear reduction model to test the effect of alkaline solution on the loading capacity of affinity chromatography packing material(MabCapture recombinant protein A affinity chromatography medium)and the disinfection interval.The research results showed that after 25 hours of disinfection,the DBC(loading capacity)could still reach over 95％.When the affinity chromatography disinfection interval was 3 cycles,the dynamic binding loading capacity of the filler decreased by 5.2％,with a small decrease,and the recovery rate did not decrease.Therefore,the disinfection time can be set at 25 hours,and the maximum disinfection interval for affinity chromatography can be set at 3 cycles.关键词
亲和层析填料/消毒时长/消毒间隔Key words
affinity chromatography packing material/disinfection duration/disinfection interval引用本文复制引用
张蕙..消毒时长及间隔对亲和层析填料(MabCapture重组蛋白A)载量的影响研究[J].工业微生物,2025,55(1):81-83,3.
