工业微生物2025，Vol.55Issue(1)：253-256,4.DOI:10.3969/j.issn.1001-6678.2025.01.065
HPLC法测定复方锌铁钙口服溶液中总葡萄糖酸根含量
Determination of Total Gluconate Acid Radical in Compound Zinc,Iron and Calcium Oral Solution by HPLC
摘要
Abstract
The paper established an HPLC method for the determination of total gluconate acid radical in compound zinc,iron and calcium oral solution.The chromatographic column was Ultimate XB NH2(250 mm×4.6 mm,5 μm).The mobile phase was 0.02 mol/L potassium dihydrogen phosphate solution-acetonitrile(40∶60).The detection wavelength was 200 nm,the flow rate was 1.0 mL/min,the injection volume was 20 μL,and the column temperature was 30 ℃.With this method,the lactic acid and gluconic acid could be effectively separated,and negative samples did not interfere with the determination of gluconate acid radical.Gluconate had a good linear relationship in the the mass concentration of 130.36-651.8 μg/mL(r=0.999).The average recovery rate was 101.34％(SRSD is 0.96％).This method had strong specificity,simple operation,high sensitivity and good repeatability,and can accurately determine the total gluconate acid radical in compound zinc,iron and calcium oral solution.关键词
HPLC/复方锌铁钙口服溶液/葡萄糖酸根Key words
HPLC/compound zinc,iron and calcium oral solution/gluconate acid radical引用本文复制引用
李琦,刘雁鸣,李钰鑫,李晓燕..HPLC法测定复方锌铁钙口服溶液中总葡萄糖酸根含量[J].工业微生物,2025,55(1):253-256,4.基金项目
湖南省自然科学基金科药联合基金(编号:2022JJ80009). （编号:2022JJ80009）
