贵州农业科学2025，Vol.53Issue(2)：22-28,7.DOI:10.3969/j.issn.1001-3601.2025.02.003
不同培养基配方对甘薯愈伤形成及生根的影响
Effects of Different Medium Formulations on Callus Formation and Rooting of Sweet Potato
摘要
Abstract
[Objective]The effects of different medium formulations on the callus formation and rooting of sweet potato were investigated to provide the technical references for tissue culture of sweet potato.[Method]The leaves or stem segments of six sweet potato varieties(Ziyun red core sweet potato,Fushu 24,Fushu 404,etc.)were treated with different disinfection methods and cultured on different medium formulations.The contamination rate,browning rate,callus induction rate,differentiation rate and rooting rate of sweet potato explants were statistically analyzed to screen suitable explant sites,disinfection methods,and suitable medium formulations for each stage of sweet potato tissue culture.[Result]The survival rate of leaves was high when sterilized with 0.1% HgCl2 for 8-10 minutes.The survival rate of stem segments was high when sterilized with 0.1% HgCl2 for 12-15 minutes.The induction rate of callus in sweet potato leaves was significantly higher than that in stem segments.Under MS+0.1 mg/L NAA+0.5 mg/L 6-BA medium,Ziyun red core sweet potato,Fushu 24,Fushu 404,Yushu 27 and Qiancai 1 had a low browning rate and high callus induction rate,while Pushu 32 had a same result under MS+1.0 mg/L NAA+0.5 mg/L 6-BA medium.Ziyun red core sweet potato could induce differentiation on MS+0.2 mg/L NAA+0.5 mg/L 6-BA medium,with the differentiation rate of 1.67% .Yushu 27 had a differentiation rate of 3.33% on MS+2.0 mg/L KT+0.5 mg/L IAA medium,the callus of other four varieties had not differentiated.The rooting rate of six sweet potato varieties on MS+1.0 mg/L 6-BA+0.3 mg/L IBA medium was high,among which the rooting rate of Ziyun red core sweet potato,Pushu 32 and Yushu 27 could reach 100.0% .[Conclusion]The optimal sterilization time for the surface of sweet potato leaves is 8-10 minutes with 0.1% HgCl2,while the optimal sterilization time for the surface of stem segments is 12-15 minutes with 0.1% HgCl2.The induction rate of callus in sweet potato leaves is significantly higher than that in stem segments,and MS+1.0 mg/L 6-BA+0.5 mg/L NAA medium has the best effect,with the callus induction rate above 97.8% .The medium suitable for adventitious bud rooting of sweet potato is MS+1.0 mg/L 6-BA+0.3 mg/L IBA,and the rooting rate is more than 92% .关键词
甘薯/培养基/MS/NAA/6-BA/愈伤组织/生根Key words
sweet potato/medium/MS/NAA/6-BA/callus/rooting分类
农业科技引用本文复制引用
范建新,邓仁菊,王维凤..不同培养基配方对甘薯愈伤形成及生根的影响[J].贵州农业科学,2025,53(2):22-28,7.基金项目
贵州省高等学校贵州山地特色园艺作物分子育种与品种创制重点实验室(黔教技[2022]053) （黔教技[2022]053）
凯里学院横向课题(HX202117) （HX202117）
凯里学院博士启动项目(BS20220101) （BS20220101）
贵州省甘薯工程技术研究中心(黔科合平台人才[2019]5201) （黔科合平台人才[2019]5201）
