贵州农业科学2025，Vol.53Issue(2)：59-66,8.DOI:10.3969/j.issn.1001-3601.2025.02.008
低纬高原不同种植模式紫色辣椒果实纤维素合成关键基因的挖掘
Discovery of Key Genes Related to Cellulose Synthesis in Purple Chili Fruits Under Different Planting Modes in Low Latitude Plateau
摘要
Abstract
[Objective]The key genes related to cellulose synthesis in purple chili fruits under two planting modes in the habitats of low latitude plateau were explored to provide a reference for studying the cellulose synthesis mechanism of purple chili fruits and selection of purple chili planting mode.[Method]The cellulose content of purple Capsicum annuum 16HN01 fruits was determined by spectrophotometry at six fruit development stages and the key genes related to cellulose synthesis and the planting mode were discovered by transcriptome sequencing,quantitative real-time fluorescence PCR(qRT-PCR)and correlation analysis.[Result]The cellulose content of purple C.annuum 16HN01 fruits in field and greenhouse modes gradually increased with fruit development process and was 3.79%-12.06% and 2.35%-9.55% respectively.The cellulose content of purple C.annuum 16HN01 fruits in greenhouse significantly decreased by 17.90%-38.09% compared with that in field.The transcriptional level of SGT,CesA2,CesA7,CSL-D3,CSL-G2,NAC,BES1 and MYB48 in purple C.annuum 16HN01 fruits planted in greenhouse was 17.13%,20.55%,17.73%,47.62%,33.68%,26.97%,42.00% and 29.00% lower than in field respectively.The transcriptional level of Korrigan gene(Kor)in purple C.annuum 16HN01 fruits planted in greenhouse was 64.94% higher than in field.The CC content of purple C.annuum 16HN01 fruits planted in field was significantly and positively related to CesA2,CSL-D3 and CSL-G2 and their correlation coefficients were 0.903,0.878 and 0.844 separately.The CC content of purple C.annuum 16HN01 fruits planted in greenhouse was positively related to CesA2 significantly and the correlation coefficients was 0.854.The CC content of purple C.annuum 16HN01 fruits planted in field was positively related to NAC and BES1 and their correlation coefficients were 0.560 and 0.413 respectively.The CC content of purple C.annuum 16HN01 fruits planted in greenhouse was positively related to NAC and the correlation coefficient was 0.671.[Conclusion]The structural gene(SG)and transcription factor gene(TF)related to cellulose synthesis of purple C.annuum fruits planted in field are mainly CesA2,CSL-D3 and CSL-G2,and NAC and BES1 in low latitude plateau respectively.The structural gene(SG)and transcription factor gene(TF)related to cellulose synthesis of purple C.annuum fruits planted in greenhouse are mainly CesA2,CSL-G2 and SGT,and NAC separately.The greenhouse planting mode can decrease cellulose content of purple C.annuum fruits and transcriptional level of the genes(except for Kor)related to cellulose synthesis.关键词
低纬高原/种植模式/紫色辣椒/果实/纤维素合成相关关键基因/挖掘Key words
low latitude plateau/planting mode/purple chili/fruit/key genes related to cellulose synthesis/discovery分类
农业科技引用本文复制引用
张丹丹,张雪廷,赵水灵,王绍祥,解志强,王文丽,赵鹏宇,袁恩平,赵昶灵..低纬高原不同种植模式紫色辣椒果实纤维素合成关键基因的挖掘[J].贵州农业科学,2025,53(2):59-66,8.基金项目
文山壮族苗族自治州农业科学院资助项目"丘北辣椒花色苷合成对产量负效应的数字化表征"(HZ2021-01) （HZ2021-01）
云南省重大科技专项计划项目"云南特异辣椒资源高效利用研究"(202202AE090031) （202202AE090031）
