海南医科大学学报2025，Vol.31Issue(5)：360-372,13.DOI:10.13210/j.cnki.jhmu.20241120.001
姜黄素调控铁死亡干预帕金森病的网络药理学研究及实验验证
Network pharmacological study and experimental verification of curcumin regulating ferroptosis in Parkinson's disease
摘要
Abstract
Objective:To explore the potential targets and mechanisms by which curcumin regulates ferroptosis in the interven-tion of Parkinson's disease(PD)based on network pharmacology,molecular docking,and experimental validation.Methods:Cur-cumin targets were obtained using databases such as Swiss Target Prediction and SEA.Therapeutic targets for PD were sourced from the GeneCards database.Common targets were identified using Venn online tools,and core targets and networks were ana-lyzed with Cytoscape plugins.Enrichment analysis was performed using the DAVID database,with visualization via microbioinfor-matics platforms.Ferroptosis-related genes were retrieved from the FerrDb database.An integrated analysis of common targets among curcumin,PD,and ferroptosis was conducted,predicting potential therapeutic targets.A C17.2 PD model was established using rotenone;the optimal concentration of curcumin was determined via CCK-8 assay.ROS,GSH,ferrous ion levels,and GPx and SOD activities were measured using kits,while RT-qPCR and Western blot analyses assessed NFE2L2 and NOX4 gene and protein expressions,respectively.Results:Network pharmacology identified 17 potential targets,including NOX4 and NFE2L2,through which curcumin may regulate ferroptosis in PD by mediating oxidative stress-related signaling pathways.In vi-tro experiments demonstrated that 2.0 μmol/L curcumin significantly reduced ROS and ferrous ion levels(P<0.01),increased GSH content(P<0.01),and enhanced GPx and SOD activities(P<0.05),while inhibiting NOX4 gene and protein expression(P<0.05)and promoting Nrf2 gene and protein expression(P<0.05).Conclusion:Curcumin may improve oxidative stress and ferroptosis in rotenone-induced C17.2 cells by regulating Nrf2 and NOX4,thereby protecting neural stem cells and improving PD outcomes.关键词
帕金森病/姜黄素/铁死亡/网络药理学/Nrf2/NOX4Key words
Parkinson's disease/Curcumin/ferroptosis/network pharmacology/Nrf2/NOX4分类
中医学引用本文复制引用
吴春阳,欧阳竞锋..姜黄素调控铁死亡干预帕金森病的网络药理学研究及实验验证[J].海南医科大学学报,2025,31(5):360-372,13.基金项目
This study was supported by the Science and Technology Innovation Project of China Academy of Chinese Medical Sciences(CI2021A00602) 中国中医科学院科技创新工程资助项目(CI2021A00602) （CI2021A00602）
