河北中医药学报2025，Vol.40Issue(1)：7-13,25,8.
基于粪菌移植验证当归芍药散对伪无菌大鼠"肠道菌群-肠黏膜机械屏障"的调节作用
Verification of Regulatory Effect of Danggui Shaoyao San on"Gut Microbiota-Intestinal Mucosal Mechanical Barrier"in Pseudo-germfree Rats Based on Fecal Microbiota Transplantation
摘要
Abstract
Objective:To use fecal microbiota transplantation(FMT)technology to verify the mechanism of Danggui Shaoyao San in regulating lipid metabolism and protecting the liver.Methods:Fifty SD rats were divided into a control group,a metabolic-associated fatty liver disease(MAFLD)group,and low,medium,and high dose groups of Danggui Shaoyao San.Except the control group,the other groups were fed with high-fat feed for 8 weeks to establish the MAFLD model,and each dosage group of Danggui Shaoyao San was trea-ted the corresponding does of the drug by intragastric administration for 4 weeks.After treatment,feces were collected to prepare fecal bacterial solution.Another 60 SD rats were randomly divided into a control group,an MAFLD rat fecal microbiota transplantation group(MO-FMT group),a normal rat fecal microbiota transplantation group(C-FMT group),and low,medium,and high dose Danggui Shaoyao San rat FMT groups.Except for the control group,the other groups were given antibiotic-free water for 4 weeks to establish a pseudo-germfree rat model.After the model was successfully established,the rats in each group were given corresponding fecal bacterial solution intervention for 4 weeks.Serum,liver tissue,ileum and colon tissues,and feces were collected from the rats in each group to de-tect related indicators.Results:Compared with the control group,the levels of serum total cholesterol(TC),triglycerides(TG),low-density lipoprotein(LDL-C),alanine aminotransferase(ALT),aspartate aminotransferase(AST),tumor necrosis factor-α(TNF-α),as well as the content or activity of TC,TG,and free fatty acids(FFA)in the liver tissue in the MO-FMT group were all increased(P<0.05).A small number of inflammatory cell infiltrations were visible in the hepatocytes.The content of serum high-density lipoprotein(HDL-C)and interleukin-10(IL-10),the α-diversity index,the abundance of Bacteroidetes,and the protein expression levels of occlu-din and ZO-1 in the intestine were all decreased(P<0.05),while the abundance of Firmicutes,the F/B value,and the protein expres-sion levels of Toll-like receptor 4(TLR4)and phosphorylated nuclear factor-κB(p-NF-κB)in the liver tissue were all increased(P<0.05).Compared with the MO-FMT group,the hepatocytes in each FMT group of Danggui Shaoyao San showed no obvious damage,and both liver function and lipid metabolism were tending towards normal(P<0.05),up-regulate the content of serum IL-10,the α-diversity index,the abundance of Bacteroidetes,and the protein expression levels of occludin and ZO-1(P<0.05),and down-regulate the content of serum TNF-α,the abundance of Firmicutes,the F/B value,and the protein expression levels of TLR4 and p-NF-κB in the liver tissue(P<0.05).Conclusion:Danggui Shaoyao San exerts its lipid-regulating and liver-protecting effects by reshaping the abundance of in-testinal microbiota,improving the function of intestinal mucosal mechanical barrier,and inhibiting the TLR4 inflammatory pathway.关键词
当归芍药散/粪菌移植/肠道菌群/肠黏膜机械屏障/TLR4/炎症反应Key words
Danggui Shaoyao San/fecal microbiota transplantation/intestinal microbiota/intestinal mucosal mechanical barrier/TLR4/inflammatory response分类
医药卫生引用本文复制引用
顾颖,高雅,张誉方,沈浩然,魏子恒,李丹彤,彪雅宁,相聪坤..基于粪菌移植验证当归芍药散对伪无菌大鼠"肠道菌群-肠黏膜机械屏障"的调节作用[J].河北中医药学报,2025,40(1):7-13,25,8.基金项目
河北省自然科学基金面上项目(H2020423028) （H2020423028）
河北省研究生创新资助项目(CXZZBS2023139) （CXZZBS2023139）
河北省高等学校科学研究项目(BJ2025232) （BJ2025232）
河北省中医药管理局科研计划项目(2025018) （2025018）
