黑龙江农业科学Issue(3)：7-11,5.DOI:10.11942/j.issn1002-2767.2025.03.0007
利用SSR技术鉴定玉米杂交种乡糯4的种子纯度
Identification of Seed Purity of Edible Maize Hybrid Xiangnuo 4 Using SSR Technology
摘要
Abstract
Seed purity is related to crop yield,quality and disease resistance,which is crucial for agricultural sustainability and market access.Xiangnuo 4 is a high-yield and high-quality fresh maize hybrid successfully selected and bred by Heilongjiang Academy of Agricultural Sciences in 2023.In order to promote molecular marker assisted breeding of fresh maize in Heilongjiang Province,in this study,Xiangnuo 4 maize hybrid and its parents,the close variety Xiangnuo 7 hybrid combination were used as materials.And from 40 pairs of maize SSR core primers,two pairs of primers,bnlg1671y17 and umc1936k4 were screened,which had obvious polymorphism and good repeatability between the parents of Xiangnuo 4,were used for purity identification.The results showed that the purity of Xiangnuo 4 using SSR molecular markers was 98.96％,and the purity of Xingnuo 4 in field plots was 96.97％,which was slightly lower than that using SSR molecular markers.SSR molecular markers can increase accuracy,reliability,and practicality for quickly and effectively identifying the purity of hybrid variety Xiangnuo 4 in production.关键词
玉米/SSR标记/纯度鉴定/杂交种Key words
maize/SSR marker/purity identification/hybrids引用本文复制引用
王然,隋冬华,张冬雪,武新娟,高佳缘,李鑫,唐贵..利用SSR技术鉴定玉米杂交种乡糯4的种子纯度[J].黑龙江农业科学,2025,(3):7-11,5.基金项目
黑龙江省农业科学院科技创新跨越工程农业特色产业项目(CX23TS02). （CX23TS02）
