黑龙江农业科学Issue(3)：39-45,7.DOI:10.11942/j.issn1002-2767.2025.03.0039
秋子梨果实着色过程花青素含量及相关基因表达分析
Analysis of Anthocyanin Content and Related Gene Expression During Fruit Coloring of Pyrus ussuriensis Maxim.
摘要
Abstract
In order to clarify the anthocyanin content in two Pyrus ussuriensis Maxim.fruits at different developmental stages,the accumulation trends of the two main components,cyanidin-3-O-glucoside and cyanidin-3-O-galactoside,as well as the expression levels of anthocyanin-related genes.In this study,the contents of anthocyanins and two main components of 5 growing fruits of'Longxiang'and'79-1'Pyrus ussuriensis Maxim.were determined,and the relationship between anthocyanins and main components and related genes were analyzed.The results showed that the anthocyanin content of'79-1'was higher than that of'Longxiang'during the fruit development of the two Pyrus ussuriensis Maxim.varieties,and the anthocyanin content reached the highest 100 days after flowering The content of two main anthocyanins in the fruit showed an overall trend of first increasing and then decreasing during the fruit development stage,and content reached a peak at 100 days after flowering.The relative expression of anthocyanin synthesis genes PbMYB108,PbMYC2,PbbHLH130 and PbbHLH62 showed a trend of first rising and then falling throughout the whole development period of fruit,which was the same as that of the two main components of anthocyanin.In addition to PbMYC2,the expression levels of three other transcriptional regulatory genes,PbbHLH130,PbbH62,and PbMYB108,were lower in'79-1'than in'Longxiang'at 40 days after flowering The results of correlation analysis showed that the expression level of PbMYC2 was not significantly correlated with the anthocyanin content,while the coefficients between PbMYB108 and PbbHLH62 and anthocyanin content were greater than 0.5 in both varieties,that they play an important role in anthocyanin synthesis.关键词
秋子梨/'龙香'/'79-1'/果实发育/花青素/基因表达Key words
Pyrus ussuriensis Maxim./'Longxiang'/'79-1'/fruit development/anthocyanin/gene expression引用本文复制引用
刘畅,任爱华,冷春旭,顾广军,胡颖慧,杨悦,韩继龙..秋子梨果实着色过程花青素含量及相关基因表达分析[J].黑龙江农业科学,2025,(3):39-45,7.基金项目
黑龙江省科研业务费(CZKYF2022-1-B030). （CZKYF2022-1-B030）
