中医药导报2025，Vol.31Issue(2)：1-6,29,7.DOI:10.13862/j.cn43-1446/r.2025.02.001
基于血清代谢组学分析哮喘宁治疗心理应激哮喘的作用机制
Effects of Xiaochuanning(哮喘宁)on the Mechanism of Action in Treating Psychological Stress-Related Asthma Based on Serum Metabolomics Analysis
摘要
Abstract
Objective:To explore the changes in serum metabolites in mice with psychological stress-related asthma(PSA)and the mechanism of action of Xiaochuanning in treating PSA based on untargeted metabolomics technology.Methods:Totally 32 SPF-level male BALB/c mice were distributed into four distinct clusters at random,including control group,asthma group,PSA group,and Chinese medicine group.Except for the control group,all other groups of mice received an intraperitoneal administration of a concoction containing ovalbumin and aluminum hydroxide on the 1st and 8th days.Subsequently,from the 15th day,they were exposed to an aerosol of 1％ovalbumin solution to induce the asthma model.The PSA and Chinese medicine groups received restraint stress from the 1st day for 28 consecutive days to establish the PSA model.The Chinese medicine group was administered Xiaochuanning continuously from 15th day for 14 days.Non-invasive lung function tests and open field tests were performed on 28th day.Samples of lung tissue and blood serum were gathered.Lung tissue was used for disease investigation with HE,and blood serum was analyzed to identify small-molecule metabolites using ultra-performance liquid chromatography linked with mass spectrometry(UPLC-MS).Principal component analysis(PCA)and partial least squares-discriminant analysis(PLS-DA)were used to screen differential metabolites and analyze metabolic pathways.Results:Compared with the asthma group,the PSA group exhibited significant pulmonary inflammatory cell infiltration,increased specific airway resistance(sRAW),decreased expiratory flow at 50％tidal volume(EF50),reduced total movement distance,and movement speed in the open field test,and significant separation of endogenous metabolites.A total of 89 differential metabolites were identified,mainly enriched in arginine biosynthesis,β-alanine metabolism,unsatu-rated fatty acid biosynthesis,arginine and proline metabolism,and GABA ergic synapse metabolic pathways.Compared with the PSA group,the Chinese medicine group showed improvements in airway inflammation,lung function(sRAW,EF50),total movement distance,and movement speed in the open field test.A total of 50 differential metabolites were identified,mainly enriched in tryptophan metabolism,taste transduction,synaptic vesicle cycle,phenylalanine,tyrosine,and tryptophan biosynthesis metabolic pathways.Conclusion:Endogenous metabolites and metabolic pathways in PSA mice are altered.Xiaochuanning can ameliorate some of the differential metabolites and disrupted metabolic pathways,thereby improving asthma symptoms and depressive states.关键词
心理应激哮喘/哮喘宁/代谢组学/超高效液相色谱-质谱法(UPLC-MS)/小鼠Key words
psychological stress-induced asthma/Xiaochuanning/metabolomics/ultra-performance liquid chromatography-mass spectrometry(UPLC-MS)/mice分类
中医学引用本文复制引用
程思益,黄贵锐,张诗瑜,崔红生..基于血清代谢组学分析哮喘宁治疗心理应激哮喘的作用机制[J].中医药导报,2025,31(2):1-6,29,7.基金项目
国家自然科学基金面上项目(82274497) （82274497）
