中医药导报2025，Vol.31Issue(2)：7-12,6.DOI:10.13862/j.cn43-1446/r.2025.02.002
基于代谢组学研究蒙药胡日查-6干预豚鼠过敏性鼻炎的作用机制
Study on the Mechanism of Mongolian Medicine Huricha-6 on Guinea Pigs with Allergic Rhinitis Based on Metabolomics
摘要
Abstract
Objective:To reveal the possible mechanism of Huricha-6 in the treatment of allergic rhinitis(AR)through the study of plasma metabolomics in guinea pigs and to verify it experimentally.Methods:The 54 SPF grade guinea pigs were randomly divided into blank control group(n=12),model group(n=14),Mongolian medicine group(n=14)and positive control group(n=14).Except the blank control group,the AR guinea pig model of the other groups was induced by ovalbumin and treated with corresponding drugs for 6 weeks.The plasma metabolites of the guinea pigs in each group were detected by UHPLC-OE-MS.The differential metabolites were screened by multivariate statistical analysis,projected importance value(VIP)and P-value,and the metabolic pathways were analyzed.Then,the metabolic pathway analysis was carried out.Western blotting was used to detect the expressions of TNF-α,ASM and NF-κB p65 proteins in lung tissues of guinea pigs in each group.Results:Compared with the blank control group,85 different metabolites were identified in the model group.Compared with the model group,33 kinds of differential metabolites were identified in Mongolian medicine group,and the differential metabolites were mainly concentrated in 32 metabolic pathways such as choline metabolism,glycerol phospholipid metabolism and sphingolipid signaling pathway.A total of 81 different metabolites were identified in positive control group,mainly concentrated in 43 pathways such as bile metabolism in cancer,glycerol phospholipid metabolism,and pyrimidine metabolism.According to the combination of network pharmacology and metabolomics KEGG pathway,9 pathways were obtained after the intersection of the two pathways.The model group showed higher expressions of TNF-α,ASM and NF-κB p65 in lung tissue than blank control group(P＜0.01).The Mongolian medicine group and positive control group showed lower expressions of TNF-α,ASM and NF-κB p65 protein than model group(P＜0.05).Conclusion:Huricha-6 may interfere with AR by affecting sphingolipid signaling pathway.关键词
过敏性鼻炎/胡日查-6/蒙药/豚鼠/血浆代谢组学/作用机制Key words
allergic rhinitis/Huricha-6/Mongolian medicine/guinea pig/plasma metabolomics/mechanism of action分类
中医学引用本文复制引用
苏日古格,乌日柴夫,吉日木巴图,李花,韩额尔德木图,孟永梅..基于代谢组学研究蒙药胡日查-6干预豚鼠过敏性鼻炎的作用机制[J].中医药导报,2025,31(2):7-12,6.基金项目
国家民委-教育部蒙医药研发工程重点实验室开放基金项目(MDK2020015) （MDK2020015）
内蒙古医科大学校级重点项目(YKD2024ZD008) （YKD2024ZD008）
