中医药导报2025，Vol.31Issue(2)：13-18,6.DOI:10.13862/j.cn43-1446/r.2025.02.003
黄芩苷通过调控铁死亡减轻H9c2细胞缺氧复氧损伤及其机制研究
Effect and Mechanism of Baicalin on Hypoxia-Reoxygenation Injury in H9c2 Cells Via Regulating Ferroptosis
摘要
Abstract
Objective:To investigate the effect and mechanism of baicalin on hypoxia-reoxygenation injury in H9c2 cells via regulating ferroptosis.Methods:Rat H9c2 cardiomyocytes hypoxia-reoxygenation injury model was constructed with Na2S2O4,followed by baicalin intervention.Cell viability,ROS,mitochondrial membrane potential,and Fe2+levels were measured using assay kits.Western blotting was utilized to evaluate cell PTGS2,NOX2,and GPX4 protein expression levels.Cell Fe2+levels were examined using assay kits after baicalin intervention with PTGS2 agonist BUR1 following Na2S2O4 induction.Results:Baicalin up to 80 μmol/L showed no toxicity to H9c2 cells.Na2S2O4 induced ferroptosis in H9c2 cells,characterized by elevated ROS and Fe2+levels,mitochondrial membrane potential collapse,and significant decrease in GPX4 expression(P＜0.05).Baicalin intervention led to decreased ROS and Fe2+levels induced by Na2S2O4,restored mitochondrial membrane potential,markedly increased GPX4 expression(P＜0.05),and reversing the process of ferroptosis.After Na2S2O4 induction in H9c2 cells,PTGS2 and NOX4 expression significantly increased(P＜0.05),while baicalin markedly reduced the expression of PTGS2 and NOX4 induced by Na2S2O4(P＜0.05).Baicalin exhibited good binding activity with PTGS2 and NOX4,with binding energies of-10.65 and-8.82 kcal/mol,respectively.BUR1 could reverse the baicalin-inhibited Fe2+levels.Conclusion:Baicalin demonstrates a clear therapeutic effect on ferroptosis in the hypoxia-reoxygenation injury model established by Na2S2O4-induced H9c2 cells,and its treatment mechanism may be associated with the inhibition of PTGS2 and NOX4 protein expression.关键词
缺血性心肌病/黄芩苷/铁死亡/大鼠H9c2心肌细胞/Na2S2O4/PTGS2/NOX4Key words
ischemic cardiomyopathy/baicalin/ferroptosis,rat H9c2 cardiomyocytes/Na2S2O4/PTGS2/NOX4分类
中医学引用本文复制引用
李家权,许锦,谭子富,方孝俊..黄芩苷通过调控铁死亡减轻H9c2细胞缺氧复氧损伤及其机制研究[J].中医药导报,2025,31(2):13-18,6.基金项目
湖北省中医药管理局科研项目(ZY2023F121) （ZY2023F121）
