中医药导报2025，Vol.31Issue(2)：19-23,5.DOI:10.13862/j.cn43-1446/r.2025.02.004
健脾益肾方调控Notch通路对5-氟尿嘧啶诱导的骨髓功能抑制的影响
The Effect of Jianpi Yishen Formula(健脾益肾方)Regulating Notch Pathway on Bone Marrow Function in Mice after Fluorouracil Chemotherapy
摘要
Abstract
Objective:To investigate the effect of Jianpi Yishen Formula on bone marrow function in mice after fluorouracil chemotherapy.Methods:Mice were randomly divided into 6 groups(10 mice in each group),including blank control group,chemotherapy group,positive control group,low-dose Chinese medicine group,medium-dose Chinese medicine group and high-dose Chinese medicine group.The number of red blood cells,white blood cells,platelets and hemoglobin in blood samples of mice was detected by hematology analyzer in each group.The levels of hematopoietic factors(IL-3,IL-6 and EPO)in the serum of mice were analyzed by ELISA in each group.The apoptosis rate of bone marrow cells was analyzed by flow cytometry in each group.The expression levels of Notch pathway-related proteins in bone marrow cells of each group were analyzed by Western blotting.Results:Compared with the blank control group,the growth rate of mouse body mass was significantly reduced in chemotherapy group(P＜0.05).The number of blood cells and hemoglobin level signifi-cantly decreased in chemotherapy group(P＜0.05),and the levels of hematopoietic factors significantly reduced in chemotherapy group(P＜0.05).The bone marrow tissue was severely damaged,and the apoptosis rate was significantly increased in chemotherapy group(P＜0.05).The expressions of Notch 1,Notch2 and Notch3 proteins were decreased in chemotherapy group(P＜0.05).Compared with the chemotherapy group,the growth rate of mouse body mass was significantly increased in Chinese medicine groups and the positive control group(P＜0.05).The number of blood cells and hemoglobin level significantly increased in Chinese medicine groups and positive control group(P＜0.05),and the levels of hematopoietic factors significantly increased in Chinese medicine groups and positive control group(P＜0.05).The bone marrow tissue was improved,and the apoptosis rate was significantly decreased in Chinese medicine groups and positive control group(P＜0.05).The expres-sions of Notch1,Notch2 and Notch3 proteins were increased in Chinese medicine groups and positive control group(P＜0.05).Conclusion:Jianpi Yishen Formula can improve bone marrow function in mice after fluorouracil chemotherapy,and the effect is closely related to the activation of Notch signaling pathway.关键词
健脾益肾方/5-氟尿嘧啶/骨髓功能/化疗/小鼠Key words
Jianpi Yishen Formula/fluorouracil/bone marrow function/chemotherapy/mice分类
中医学引用本文复制引用
袁结文,邹铭斐,刘丽丽..健脾益肾方调控Notch通路对5-氟尿嘧啶诱导的骨髓功能抑制的影响[J].中医药导报,2025,31(2):19-23,5.基金项目
广东省名中医传承工作室建设项目(粤中医办函[2020]1号) （粤中医办函[2020]1号）
佛山市名中医传承工作室建设项目(佛卫函[2022]92号) （佛卫函[2022]92号）
佛山市"十四五"中医专病建设项目(佛卫函[2020]15号) （佛卫函[2020]15号）
广东省第三批名中医师承项目(粤中医办函[2018]5号) （粤中医办函[2018]5号）
