中医药导报2025，Vol.31Issue(2)：24-29,6.DOI:10.13862/j.cn43-1446/r.2025.02.005
健脾愈疡汤对溃疡性结肠炎模型小鼠肠黏膜屏障修复作用的研究
The Effect of Jianpi Yuyang Decoction(健脾愈疡汤)on the Repair of Intestinal Mucosal Barrier in Mice with Ulcerative Colitis
摘要
Abstract
Objective:To investigate the repair effect of Jianpi Yuyang decoction on the intestinal mucosa of ulcerative colitis(UC)model mice.Methods:After 7 days of adaptive feeding,10 of the 43 SPF male KM mice were randomly selected as the normal group,and the rest were made of modules.The mice were copied with 3％ dextran sodium sulfate solution for 14 consecutive days,to establish UC mouse model.After the modeling was completed,3 evaluation models were randomly selected.After the model was established,30 mice were randomly divided into model group,Western medicine group,and Chinese medicine group,with 10 mice in each group.The The mice were given mesalazine enteric-coated tablets by gavage in Western medicine group,while Jianpi Yuyang decoction in Chinese medicine group.And the normal group and model group were given the same dose of distilled water by gavage for 14 consecutive days.The general condition,disease activity index(DAI)score,histopathological score and intestinal mucosal permeability of the mice were observed.Western blotting was used to detect the protein expression of ZO-1 and Tricellulin in colon tissues.Results:Compared with the normal group,some mice showed symptoms of frizziness,laziness,mucus and blood in model group.The DAI score,intestinal mucosal permeability and histological scores were significantly increased in model group(P＜0.05),while the protein expressions of ZO-1 and Tricellulin were significantly decreased(P＜0.05).Compared with the model group,the DAI score,intestinal mucosal permeability and histology scores were significantly decreased in Western medicine group and Chinese medicine group(P＜0.05).The contents of ZO-1 and Tricellulin protein were significantly increased in Western medicine group(P＜0.05),and the expression of ZO-1 protein was increased in Chinese medicine group(P＜0.05),while the Tricellulin protein expression were not significantly increased in Chinese medicine group.Conclusion:Jianpi Yuyang decoction shows a certain repair effect on the intestinal mucosa of UC mice.关键词
溃疡性结肠炎/健脾愈疡汤/肠黏膜屏障/紧密连接蛋白/小鼠Key words
ulcerative colitis/Jianpi Yuyang decoction/intestinal mucosal barrierx/tight junction proteins/mice分类
医药卫生引用本文复制引用
梁巧俐,陈柏辉,陈映西,潘娜,黄晓燕..健脾愈疡汤对溃疡性结肠炎模型小鼠肠黏膜屏障修复作用的研究[J].中医药导报,2025,31(2):24-29,6.基金项目
广西岐黄学者培养项目(2022015-003-01) （2022015-003-01）
"四象脾土和五脏"脾胃治未病创新团队(2021B001) （2021B001）
广西科技厅自然科学基金面上项目(2020GXNSFAA238009) （2020GXNSFAA238009）
