中医药导报2025，Vol.31Issue(2)：30-34,40,6.DOI:10.13862/j.cn43-1446/r.2025.02.006
痰瘀互结型动脉粥样硬化大鼠病证结合模型的建立与评估
Establishment and Evaluation of Disease and Syndrome Combined Model of Phlegm-Blood Stasis Interjunction Atherosclerosis in Rats
摘要
Abstract
Objective:To explore the construction method of a rat model of atherosclerosis with phlegm-blood stasis,assess its feasibility,and provide an initial evaluation of the model,laying the groundwork for further experimental research.Methods:SD rats were selected and a model of phlegm-blood stasis intertwined atherosclerosis was established by feeding them with a high-fat diet and injecting of vitamin D3(600 000 IU/kg)in a single dose.The establishment of the sphlegm-blood stasis intertwined atherosclerosis rat model was initially evaluated through symptom evaluation,pathological observation,and biochemical index detection.Results:In the model group rats,the total score of syndrome after the experiment was higher than before(P＜0.05),and the model group showed higher total score of syndrome than blank group after the experiment(P＜0.05).The model group showed higher TG,TC,LDL-C,FIB,platelet aggregation rate,plasma viscosity,whole blood viscosity(3/s,30/s,50/s,100/s,180/s),red blood cell rigidity index,and aggregation index than blank group,while lower HDL-C,PT,INR,APTT,and TT than blank group(P＜0.05).The model group showed higher thickness of the middle layer of the thoracic aorta than that blank group(P＜0.05).According to HE staining and oil red O staining in pathological analysis,the model group showed obvious atherosclerotic pathological changes.Conclusion:This phlegm-blood stasis type atherosclerosis syndrome model combining Chinese medicine syndrome and Western medicine pathology meets the changes in the phlegm-blood stasis syndrome and the pathological and physiological characteristics of atherosclerosis,and it is similar to the expression characteristics of human atherosclerosis.The modeling method is feasible.关键词
动脉粥样硬化/痰瘀互结证/病证结合模型/大鼠Key words
atherosclerosis/phlegm-blood stasis syndrome/model of combination of disease and syndrome/rat分类
中医学引用本文复制引用
孙朝辉,李雁丹,李钦,解海雪,魏淑芳..痰瘀互结型动脉粥样硬化大鼠病证结合模型的建立与评估[J].中医药导报,2025,31(2):30-34,40,6.基金项目
云南省科技厅科技计划项目(202101AZ070001-324) （202101AZ070001-324）
云南中医药大学第一附属医院"卓越青年人才计划"项目[云中附党字(2021)5号] （2021）
云南中医药大学校园联合基金项目-重点项目(XYLH202203) （XYLH202203）
云南中医药大学基础医学院、云南省中西医结合慢病防治重点实验室(2019DG016) （2019DG016）
云南中医药大学第一附属医院、云南省中医药治未病工程研究中心(云发改高技[2023]992号) （云发改高技[2023]992号）
