中医药导报2025，Vol.31Issue(2)：35-40,6.DOI:10.13862/j.cn43-1446/r.2025.02.007
基于UPLC-Q-TOF-MS法分析何首乌不同炮制品化学成分及其抗衰老作用
The Chemical Components of Heshouwu(Polygonum multiflorum)and Different Products Based on the UPLC-Q-TOF-MS Method and Their Effects on Anti-Aging
摘要
Abstract
Objective:The composition differences and anti-oxidation and anti-aging effects of Heshouwu(Polygonum multiflorum)raw product,Beijing Traditional Chinese Medicine Preparation Standard processed product(Beijing processed product)and Pharmacopoeia of the People's Republic of China processed product(China Pharmacopoeia processed product)were analyzed.Methods:Based on the ultra-high performance liquid chromatography-tandem quadrupole time-of-flight high resolution mass spectrometry(UPLC-Q-TOF-MS)method,the components of different prepared products of Heshouwu(Polygonum multiflorum)were analyzed.DPPH free radical scavenging rate was determined to evaluate the antioxidant capacity of different prepared Heshouwu(Polygonum multiflorum)in vitro.The lipofuscin level of C.elegans was observed to analyze the effects of different products of Heshouwu(Polygonum multiflorum)on anti-aging.Results:A total of 30 different components were identified from different Heshouwu(Polygonum multiflorum)product,and 20 components were found in 3 different product.The half clearance rate of raw product,China Pharmacopoeia processed product and Beijing processed product were 0.006 9,0.043 6 and 0.033 0mg/mL respectively.The raw product group,Beijing processed product group and China Pharmacopoeia processed product group showed lower fluorescence intensity of caenorhabditiselegans than blank group(P＜0.001).Conclusion:The different products of Heshouwu(Polygonum multiflorum)have good antioxidant and anti-aging ability,and the Beijing processed product is superior to the China Pharmacopoeia processed product.关键词
何首乌/秀丽隐杆线虫/超高效液相色谱-串联四极杆飞行时间高分辨质谱/抗氧化/抗衰老Key words
Heshouwu(Polygonum multiflorum)/C.elegans/ultra-high performance liquid chromatography-tandem quadrupole time-of-flight high resolution mass spectrometry/anti-oxidation/anti-aging分类
中医学引用本文复制引用
何秀娟,王璇,黄蓉,彭若威,李晓欣,刘永刚,谭鹏..基于UPLC-Q-TOF-MS法分析何首乌不同炮制品化学成分及其抗衰老作用[J].中医药导报,2025,31(2):35-40,6.基金项目
国家"重大新药创制"科技重大专项(2018YFC1706500) （2018YFC1706500）
