中医药导报2025，Vol.31Issue(2)：41-46,56,7.DOI:10.13862/j.cn43-1446/r.2025.02.008
基于灰色关联度的保元汤基准样品抗氧化作用的谱效关系研究
Study on Spectrum-Effect Relationship of Anti-Inflammation of Baoyuan Decoction(保元汤)Based on Gray Correlation Analysis
摘要
Abstract
Objective:A spectrum-activity relationship is established with the high-performance liquid chromatography(HPLC)fingerprint of the famous classical formula Baoyuan Decoction(BYD)and the in vitro antioxidant activity,to screen the components related to antioxidant activity by combining them with spectral effect relationship analysis.Methods:The HPLC method was used to establish the fingerprints of 16 batches of BYD benchmark samples,and the multi-wavelength switching fingerprints were established based on the similarity evaluations of the control fingerprints,which were all greater than 0.9.And the fingerprint data were combined with the cluster analysis,principal component analysis,and least-square two analysis to conduct the stoichiometric study.The antioxidant activities of BYD were evaluated by the DPPH radical scavenging methods.The correlation analysis of the 16 batches of BYD common peaks with their antioxidant capacity indexes was carried out by the gray correlation method to comprehensively evaluate the screened active ingredients.Results:In the dual-wavelength fingerprints of BYD,15 peaks were identified at 203 nm,and 3 peaks were identified at 254 nm.A total of 8 compounds were recognized by the control,and the multi-wavelength switching fingerprints of BYD contained 18 peaks.The results of the cluster analysis showed that the samples could be roughly divided into 2 groups,and that the compounds corresponding to the common peaks F6,F3,F1,F2 and F15 were the main difference components.The results of the antioxidant experiment showed that all batches of benchmark samples exhibited good antioxidant activity and that the antioxidant activity was positively correlated with con-centration.In particular S8 showed the strongest antioxidant activity.Combining the results of gray correlation analysis and comprehensive scoring,the compounds corresponding to the common peaks F15,F7,F9,F4,and F5 may be the most important components in characterizing the antioxidant activity of BYD.Conclusion:The HPLC fingerprints of BYD can be used for the quality evaluation of the formula,and the study of the spectral effect relationship to its antioxidant activity provides a reference for the Q-marker screening and quality con-trol of BYD.关键词
保元汤/经典名方/指纹图谱/谱效关系/抗氧化/灰色关联分析Key words
Baoyuan decoction/famous classical formula/fingerprinting/spectrum-effect relationship/antioxidant/gray correlation analysis分类
中医学引用本文复制引用
李思颖,金晨,刘均正,童猛辉,何颖,朱洁,谭闽怀,黄慧莲..基于灰色关联度的保元汤基准样品抗氧化作用的谱效关系研究[J].中医药导报,2025,31(2):41-46,56,7.基金项目
江西中医药大学校级科技创新团队发展计划(CXTD22002) （CXTD22002）
