中国牛业科学2025，Vol.51Issue(1)：10-14,5.
新疆褐牛遗传多样性和母系遗传背景分析
Analysis of Genetic Polymorphism and Maternal Genetic Background of Xinjiang Brown Cattle
摘要
Abstract
In order to evaluate and excavate genetic resources and germplasm resources of Xinjiang Brown Cattle,this study investigated the genetic polymorphism and maternal genetic background of Xinjiang Brown Cattle.PCR amplification and sequencing of genomic DNA were conducted based on the marker sites within mtDNA D-Loop region.And further this study analyzed the genetic polymorphism in-dexes,such as nucleotide polymorphism loci,nucleotide diversity and number of haplotype of the nucleotide sequence of mtDNA D-loop of 8 indigenous yellow cattle species and 5 introduced species as control,and constructed phylogenetic tree.The mtDNA D-loop of Xinjiang Brown cattle and its hybrid populations were rich in nucleotide A and T.The average contents of A,T,C and G were 32.75％,28.78％,24.65％and 13.82％,respectively.The average content of C+G(38.47％)was significantly lower than that of A+T(61.53％).A total of 89 polymorphic sites were identified,of which 25 were single polymorphic sites and 64 were reduced information sites.Fifty-five haplotypes were defined.Xinjiang brown cattle and their hy-brids belong to the same group as European common cattle and African Zebu cattle.Xinjiang Brown cattle and its hybrids were rich in nucleotide mutations and higher population variation degree.Xinjiang Brown cattle have diverse origins and possess common cattle and zebu cattle genetic backgrounds.关键词
新疆褐牛/遗传多样性/线粒体DNA D-Loop/系统发育Key words
Xinjiang Brown Cattle/genetic polymorphism/mtDNA D-loop/phylogeny分类
农业科技引用本文复制引用
杜玮,周振勇,叶志兵,崔繁荣,马义诚,袁理星,李红波..新疆褐牛遗传多样性和母系遗传背景分析[J].中国牛业科学,2025,51(1):10-14,5.基金项目
新疆维吾尔自治区公益性科研院所基本科研业务经费资助项目"新疆褐牛遗传多样性和母系遗传背景研究" （）
新疆维吾尔自治区肉牛产业技术体系项目(XJAR-10-08) （XJAR-10-08）
国家肉牛牦牛产业技术体系项目(CARS-37) （CARS-37）
