海洋科学2024，Vol.48Issue(12)：83-93,11.DOI:10.11759/hykx20240320002
方格星虫(Sipunculus nudus L.)体壁多糖的分离纯化、结构鉴定和抗凝血活性研究
Extraction,purification,structural determination,and anti-coagulant activity of polysaccharides from the body wall of Sipunculus nudus L.
摘要
Abstract
With an aging population,cardiovascular and cerebrovascular diseases have become common illnesses and one of the main causes of death in humans.The development of natural anticoagulant bioactive compounds is of great significance for the prevention and treatment of these diseases.Sipunculus nudus L.is a marine benthic animal mainly inhabiting the offshore areas of southern China.Its body wall is rich in bioactive substances such as poly-saccharides.However,no reports are available on its anticoagulant activity yet.Herein,a nonneutral polysaccharide was isolated and purified from the body wall of S.nudus L.The yield of S.nudus polysaccharide(SLP)was 5.76%.It mainly comprised Man,Rib,Gal,and Glc,with a molar ratio of 1∶2.83∶5.34∶6.95.The Mn of SLP was 9 201 g/mol,and the polymer dispersity index(PDI)was 1.05,indicating its high uniformity.SEM and FT-IR analysis showed that SLP exhibited an irregular lamellar structure and mainly existed in the α-pyranose configura-tion.SLP exhibited a prolonging effect on APTT,showing a dose-effect relationship at 200-400 µg/mL;however,the activity saturated at 800 µg/mL.The anticoagulant activity of SLP is still inferior to that of chondroitin sulfate and marine sulfated polysaccharides such as those from sea cucumbers,which may be related to its low sulfate content.This finding also suggests that SLP may have a different anticoagulant mechanism from other marine pol-ysaccharides,worthy of further research.In summary,this study provides new candidate substances and research avenues for developing novel anticoagulant drugs derived from marine animals.关键词
方格星虫/多糖/结构测定/抗凝血活性Key words
Sipunculus nudus L./polysaccharides/structural determination/anticoagulant activity分类
农业科技引用本文复制引用
张学鑫,李荣锋,李航,李冠天,毕允晨..方格星虫(Sipunculus nudus L.)体壁多糖的分离纯化、结构鉴定和抗凝血活性研究[J].海洋科学,2024,48(12):83-93,11.基金项目
泰山学者青年项目(E3KY20109N)Taishan Scholars Program,No.E3KY20109N （E3KY20109N）
