海洋科学2024，Vol.48Issue(12)：94-101,8.DOI:10.11759/hykx20240311002
海浪胁迫对单体牡蛎脂质代谢的影响
Mechanical stress from waves affects lipid metabolism in clutchless oysters
摘要
Abstract
Oysters are sessile bivalves that inhabit solid and stable substrata.Their dependence on the surrounding envi-ronment makes oysters highly susceptible to environmental fluctuations.However,the cage or hanging culture of clutch-less oysters used by most farms may expose them to intense and acute mechanical stress caused by waves.The rapid change in oyster plumpness in certain areas followed large storm waves,which are presumed to be related to the shock stress induced by the culture mode.However,the physiological response of free-living oysters to wave-induced mechan-ical stress is not yet clear.The mechanisms underlying the rapid change in plumpness still need to be revealed.In this study,the metabolic activity dynamics of clutchless oysters under simulated wave stress were investigated by an untar-geted metabolomics approach.Oysters were treated for 6 h in the simulation machine,and serum was obtained from the samples collected immediately,6,and 18 h after treatment,together from individuals without treatment.A total of 447 differential metabolites were identified from all three treatment groups by analyzing the mass spectrometric data.KEGG metabolic pathway enrichment indicated that the stress mainly induced changes in the metabolites involved in the metab-olism pathways of lipids such as glycerophospholipid,sphingolipid,linoleic acid,and arachidonic acid.The glycerol es-ter hydrolysis products changed markedly,suggesting the induction of a series of stress responses and pathophysiological processes after wave stress.The results provided a reference for elucidating the effects of storm waves on cultured clutchless oysters and laid a foundation for solving the industrial problem of rapid changes in oyster plumpness.关键词
长牡蛎/海浪胁迫/非靶向代谢组学/甘油磷脂Key words
Crassostrea gigas/wave stress/untargeted metabolomics/glyceryl phosphatide分类
农业科技引用本文复制引用
于海洋,孟杰,许飞..海浪胁迫对单体牡蛎脂质代谢的影响[J].海洋科学,2024,48(12):94-101,8.基金项目
国家重点研发计划(2022YFD2401203) （2022YFD2401203）
泰山学者青年专家项目(tsqn202211250)National Key R&D Program of China,No.2022YFD2401203 （tsqn202211250）
Taishan Scholars Program,No.tsqn202211250 （）
